PMID- 10521509
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 43
DP  - 1999 Oct 22
TI  - Regulators of G protein signaling 6 and 7. Purification of complexes with gbeta5 
      and assessment of their effects on g protein-mediated signaling pathways.
PG  - 31087-93
AB  - Regulators of G protein signaling (RGS) proteins that contain DEP (disheveled,
      EGL-10, pleckstrin) and GGL (G protein gamma subunit-like) domains form a
      subfamily that includes the mammalian RGS proteins RGS6, RGS7, RGS9, and RGS11.
      We describe the cloning of RGS6 cDNA, the specificity of interaction of RGS6 and 
      RGS7 with G protein beta subunits, and certain biochemical properties of
      RGS6/beta5 and RGS7/beta5 complexes. After expression in Sf9 cells, complexes of 
      both RGS6 and RGS7 with the Gbeta5 subunit (but not Gbetas 1-4) are found in the 
      cytosol. When purified, these complexes are similar to RGS11/beta5 in that they
      act as GTPase-activating proteins specifically toward Galpha(o). Unlike
      conventional G(betagamma) complexes, RGS6/beta5 and RGS7/beta5 do not form
      heterotrimeric complexes with either Galpha(o)-GDP or Galpha(q)-GDP. Neither
      RGS6/beta5 nor RGS7/beta5 altered the activity of adenylyl cyclases types I, II, 
      or V, nor were they able to activate either phospholipase C-beta1 or -beta2.
      However, the RGS/beta5 complexes inhibited beta(1)gamma(2)-mediated activation of
      phospholipase C-beta2. RGS/beta5 complexes may contribute to the selectivity of
      signal transduction initiated by receptors coupled to G(i) and G(o) by binding to
      phospholipase C and stimulating the GTPase activity of Galpha(o).
FAU - Posner, B A
AU  - Posner BA
AD  - Department of Pharmacology, University of Texas Southwestern Medical Center,
      Dallas, Texas 75235, USA.
FAU - Gilman, A G
AU  - Gilman AG
FAU - Harris, B A
AU  - Harris BA
LA  - eng
GR  - GM34497/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (GTP Phosphohydrolase Activators)
RN  - 0 (Macromolecular Substances)
RN  - 0 (RGS Proteins)
RN  - 0 (RGS11 protein, human)
RN  - 0 (RGS6 protein, human)
RN  - 0 (RGS7 protein, human)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Rgs11 protein, mouse)
RN  - 0 (Rgs6 protein, mouse)
RN  - 0 (Rgs6 protein, rat)
RN  - 0 (Rgs7 protein, mouse)
RN  - 0 (regulator of g-protein signaling 9)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cloning, Molecular
MH  - GTP Phosphohydrolase Activators/*metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Humans
MH  - Kinetics
MH  - Macromolecular Substances
MH  - Mice
MH  - Molecular Sequence Data
MH  - RGS Proteins/chemistry/*metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction/*physiology
MH  - Spodoptera
MH  - Transfection
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - 10.1074/jbc.274.43.31087 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 22;274(43):31087-93. doi: 10.1074/jbc.274.43.31087.