PMID- 10521507
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 43
DP  - 1999 Oct 22
TI  - Characterization of a mammalian gene related to the yeast CCR4 general
      transcription factor and revealed by transposon insertion.
PG  - 31068-75
AB  - Murine intracisternal A-particles (IAPs) are reiterated retrovirus-like
      transposable elements that can act as insertional mutagens. Accordingly, we
      previously identified a chimeric transcript initiated at an IAP promoter and
      extending through a 3'-located open reading frame with significant similarity to 
      the C-terminal domain of the yeast CCR4 general transcription factor. In this
      report, we characterize the corresponding murine gene, mCCR4, and its human
      homologue, thus providing the first description of CCR4-like factors in mammals. 
      cDNA cloning revealed two mCCR4 mRNAs of 2.7 and 3.1 kilobases, differing by
      their transcription start sites within the native mCCR4 gene promoter, and
      encoding a putative 430-amino acid protein. The mCCR4 gene contains three exons
      and two introns spanning almost 27 kilobases. The IAP insertion, detected only in
      some laboratory mouse strains, is recent and lies within the first intron. The
      5'-region of the gene has features of housekeeping gene promoters. It lacks a
      TATA box but contains a CpG island and Sp1 sites. This region discloses strong
      promoter activity in transient transfection assays and also stimulates
      transcription in the reverse orientation, a feature common to other CpG
      island-containing promoters. Transcripts were detected in all the organs tested, 
      although at a variable level, and displayed no strain-dependent differences
      relative to the IAP insertion, suggesting the existence of mechanisms preserving 
      mCCR4 transcription from the usually deleterious effects of intronic
      transposition. The strong amino acid conservation between the human, murine, and 
      the previously identified Xenopus CCR4-like proteins, is consistent with an
      important and conserved role for this protein in vertebrates.
FAU - Dupressoir, A
AU  - Dupressoir A
AD  - Unite des Retrovirus Endogenes et Elements Retroides des Eucaryotes Superieurs,
      CNRS UMR 1573, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94805
      Villejuif Cedex, France.
FAU - Barbot, W
AU  - Barbot W
FAU - Loireau, M P
AU  - Loireau MP
FAU - Heidmann, T
AU  - Heidmann T
LA  - eng
SI  - GENBANK/AF183960
SI  - GENBANK/AF183961
SI  - GENBANK/AF183962
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 3.1.- (CCR4 protein, S cerevisiae)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA-Binding Proteins/genetics
MH  - Fungal Proteins/*genetics
MH  - *Genes, Intracisternal A-Particle
MH  - Humans
MH  - Mammals
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred DBA
MH  - Molecular Sequence Data
MH  - Mutagenesis, Insertional
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - *Ribonucleases
MH  - Saccharomyces cerevisiae/*genetics
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transcription Factors/*genetics
MH  - *Transcription, Genetic
MH  - Xenopus
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - 10.1074/jbc.274.43.31068 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 22;274(43):31068-75. doi: 10.1074/jbc.274.43.31068.