PMID- 10521485
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 43
DP  - 1999 Oct 22
TI  - nArgBP2, a novel neural member of ponsin/ArgBP2/vinexin family that interacts
      with synapse-associated protein 90/postsynaptic density-95-associated protein
      (SAPAP).
PG  - 30914-8
AB  - Postsynaptic density (PSD)-95/synapse-associated protein (SAP) 90 and synaptic
      scaffolding molecule (S-SCAM) are synaptic membrane-associated guanylate kinases.
      Both the proteins interact with SAP90/PSD-95-associated protein (SAPAP) (also
      called guanylate kinase-associated protein/Dlg-associated protein). SAPAP is a
      protein highly enriched in the PSD fraction and may link PSD-95/SAP90 and S-SCAM 
      to Triton X-100-insoluble structures. We found here a novel SAPAP-interacting
      protein, which was specifically expressed in neural tissue and was present in the
      postsynaptic density fraction in brain. This protein had a sorbin homology domain
      in the N terminus, a zinc finger motif in the middle region, and three src
      homology (SH) 3 domains in the C terminus and was homologous to the
      ponsin/ArgBP2/vinexin family proteins. We named this protein nArgBP2 because it
      was the most homologous to ArgBP2. nArgBP2 is a neural member of a growing family
      of SH3-containing proteins. nArgBP2 bound to the proline-rich region of SAPAP via
      its third SH3 domain and was coimmunoprecipitated with SAPAP from the extract of 
      rat brain. Furthermore, nArgBP2 was colocalized with SAPAP at synapses in
      cerebellum. nArgBP2 bound to not only SAPAP but also vinculin and l-afadin, known
      to bind to ponsin and vinexin. nArgBP2 may be implicated in the protein network
      around SAPAP in the PSD.
FAU - Kawabe, H
AU  - Kawabe H
AD  - Department of Molecular Biology, Osaka University Graduate School of
      Medicine/Faculty of Medicine, Suita 565-0871, Japan.
FAU - Hata, Y
AU  - Hata Y
FAU - Takeuchi, M
AU  - Takeuchi M
FAU - Ide, N
AU  - Ide N
FAU - Mizoguchi, A
AU  - Mizoguchi A
FAU - Takai, Y
AU  - Takai Y
LA  - eng
SI  - GENBANK/AF026505
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (DLG4 protein, human)
RN  - 0 (DLGAP1 protein, human)
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SAP90-PSD95 Associated Proteins)
RN  - 0 (SCAM-1 protein, human)
RN  - 0 (Sorbs2 protein, rat)
RN  - 0 (ponsin)
RN  - 0 (postsynaptic density proteins)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Brain/*metabolism
MH  - Cloning, Molecular
MH  - Disks Large Homolog 4 Protein
MH  - Gene Expression
MH  - Homeodomain Proteins/metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Membrane Proteins
MH  - Microfilament Proteins/metabolism
MH  - Molecular Sequence Data
MH  - Muscle Proteins/metabolism
MH  - Nerve Tissue Proteins/*metabolism
MH  - Organ Specificity
MH  - Rats
MH  - Recombinant Proteins/chemistry/metabolism
MH  - SAP90-PSD95 Associated Proteins
MH  - Zinc Fingers
MH  - src Homology Domains
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - 10.1074/jbc.274.43.30914 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 22;274(43):30914-8. doi: 10.1074/jbc.274.43.30914.