PMID- 10521462
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20220309
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 43
DP  - 1999 Oct 22
TI  - TNIK, a novel member of the germinal center kinase family that activates the
      c-Jun N-terminal kinase pathway and regulates the cytoskeleton.
PG  - 30729-37
AB  - Germinal center kinases (GCKs) compose a subgroup of the Ste20 family of kinases.
      Here we describe the cloning and characterization of a novel GCK family kinase,
      Traf2- and Nck-interacting kinase (TNIK) that interacts with both Traf2 and Nck. 
      TNIK encodes a polypeptide of 1360 amino acids with eight spliced isoforms. It
      has 90% amino acid identity to the Nck-interacting kinase in both the N-terminal 
      kinase domain and the C-terminal germinal center kinase homology region. The
      homology drops to 53% in the intermediate region. TNIK specifically activates the
      c-Jun N-terminal kinase pathway when transfected into Phoenix-A cells
      (derivatives of 293 cells), similar to many GCKs. However, in contrast to other
      GCKs, this activation is mediated solely by the GCK homology region of TNIK. In
      addition, in Phoenix-A, NIH-3T3, and Hela cells, overexpression of wild type
      TNIK, but not the kinase mutant form of TNIK, results in the disruption of
      F-actin structure and the inhibition of cell spreading. Furthermore, TNIK can
      phosphorylate Gelsolin in vitro. This is the first time that a GCK family kinase 
      is shown to be potentially involved in the regulation of cytoskeleton.
FAU - Fu, C A
AU  - Fu CA
AD  - Rigel, Inc., South San Francisco, California 94080, USA.
FAU - Shen, M
AU  - Shen M
FAU - Huang, B C
AU  - Huang BC
FAU - Lasaga, J
AU  - Lasaga J
FAU - Payan, D G
AU  - Payan DG
FAU - Luo, Y
AU  - Luo Y
LA  - eng
SI  - GENBANK/AF172264
SI  - GENBANK/AF172265
SI  - GENBANK/AF172266
SI  - GENBANK/AF172267
SI  - GENBANK/AF172268
SI  - GENBANK/AF172269
SI  - GENBANK/AF172270
SI  - GENBANK/AF172271
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Germinal Center Kinases)
RN  - 0 (Nck protein)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Recombinant Proteins)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (TNIK protein, human)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - 3T3 Cells
MH  - Adaptor Proteins, Signal Transducing
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/enzymology
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cytoskeleton/*physiology
MH  - Gene Library
MH  - Germinal Center/*physiology
MH  - Germinal Center Kinases
MH  - HeLa Cells
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Molecular Sequence Data
MH  - Oncogene Proteins/metabolism
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Proteins/metabolism
MH  - Receptors, Tumor Necrosis Factor/metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - TNF Receptor-Associated Factor 2
MH  - Transfection
MH  - src Homology Domains
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - 10.1074/jbc.274.43.30729 [doi]
AID - S0021-9258(19)51742-2 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 22;274(43):30729-37. doi: 10.1074/jbc.274.43.30729.