PMID- 10521354
OWN - NLM
STAT- MEDLINE
DCOM- 19991108
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 286
IP  - 5439
DP  - 1999 Oct 15
TI  - Yeast gene for a Tyr-DNA phosphodiesterase that repairs topoisomerase I
      complexes.
PG  - 552-5
AB  - Covalent intermediates between topoisomerase I and DNA can become dead-end
      complexes that lead to cell death. Here, the isolation of the gene for an enzyme 
      that can hydrolyze the bond between this protein and DNA is described.
      Enzyme-defective mutants of yeast are hypersensitive to treatments that increase 
      the amount of covalent complexes, indicative of enzyme involvement in repair. The
      gene is conserved in eukaryotes and identifies a family of enzymes that has not
      been previously recognized. The presence of this gene in humans may have
      implications for the effectiveness of topoisomerase I poisons, such as the
      camptothecins, in chemotherapy.
FAU - Pouliot, J J
AU  - Pouliot JJ
AD  - Laboratory of Molecular Biology, National Institute of Mental Health, Building
      36, Room 1B08, Bethesda, MD 20892-4034, USA.
FAU - Yao, K C
AU  - Yao KC
FAU - Robertson, C A
AU  - Robertson CA
FAU - Nash, H A
AU  - Nash HA
LA  - eng
SI  - GENBANK/AF182002
SI  - GENBANK/AF182003
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (DNA, Fungal)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.1.4.- (TDP1 protein, human)
RN  - EC 3.1.4.- (tyrosyl-DNA phosphodiesterase)
RN  - EC 5.99.1.2 (DNA Topoisomerases, Type I)
RN  - XT3Z54Z28A (Camptothecin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Camptothecin/pharmacology
MH  - *DNA Repair
MH  - DNA Topoisomerases, Type I/genetics/*metabolism
MH  - DNA, Fungal/*metabolism
MH  - Expressed Sequence Tags
MH  - Genes, Fungal
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Phosphoric Diester Hydrolases/chemistry/*genetics/metabolism
MH  - Saccharomyces cerevisiae/drug effects/enzymology/*genetics
MH  - Sequence Alignment
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - 7897 [pii]
AID - 10.1126/science.286.5439.552 [doi]
PST - ppublish
SO  - Science. 1999 Oct 15;286(5439):552-5. doi: 10.1126/science.286.5439.552.