PMID- 10520990
OWN - NLM
STAT- MEDLINE
DCOM- 19991025
LR  - 20220330
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 99
IP  - 1
DP  - 1999 Oct 1
TI  - A novel role for 3-O-sulfated heparan sulfate in herpes simplex virus 1 entry.
PG  - 13-22
AB  - Herpes simplex virus type 1 (HSV-1) binds to cells through interactions of viral 
      glycoproteins gB and gC with heparan sulfate chains on cell surface
      proteoglycans. This binding is not sufficient for viral entry, which requires
      fusion between the viral envelope and cell membrane. Here, we show that heparan
      sulfate modified by a subset of the multiple D-glucosaminyl 3-O-sulfotransferase 
      isoforms provides sites for the binding of a third viral glycoprotein, gD, and
      for initiation of HSV-1 entry. We conclude that susceptibility of cells to HSV-1 
      entry depends on (1) presence of heparan sulfate chains to which virus can bind
      and (2) 3-O-sulfation of specific glucosamine residues in heparan sulfate to
      generate gD-binding sites or the expression of other previously identified
      gD-binding receptors.
FAU - Shukla, D
AU  - Shukla D
AD  - Department of Microbiology-Immunology, Northwestern University Medical School,
      Chicago, Illinois 60611, USA.
FAU - Liu, J
AU  - Liu J
FAU - Blaiklock, P
AU  - Blaiklock P
FAU - Shworak, N W
AU  - Shworak NW
FAU - Bai, X
AU  - Bai X
FAU - Esko, J D
AU  - Esko JD
FAU - Cohen, G H
AU  - Cohen GH
FAU - Eisenberg, R J
AU  - Eisenberg RJ
FAU - Rosenberg, R D
AU  - Rosenberg RD
FAU - Spear, P G
AU  - Spear PG
LA  - eng
SI  - GENBANK/AF168992
GR  - R01 AI 36293/AI/NIAID NIH HHS/United States
GR  - R01 HL 59479/HL/NHLBI NIH HHS/United States
GR  - U01 AI 31494/AI/NIAID NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein B, Simplexvirus)
RN  - 0 (glycoprotein D, Human herpesvirus 1)
RN  - 0 (glycoprotein gC, herpes simplex virus type 1)
RN  - 9050-30-0 (Heparitin Sulfate)
SB  - IM
MH  - Amino Acid Substitution
MH  - Animals
MH  - CHO Cells/chemistry/metabolism/virology
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Disease Susceptibility
MH  - Heparitin Sulfate/*genetics/*metabolism
MH  - Herpes Simplex/*physiopathology
MH  - Herpesvirus 1, Human/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Plasmids
MH  - Protein Binding
MH  - Sequence Homology, Amino Acid
MH  - Transfection
MH  - Viral Envelope Proteins/*metabolism
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - S0092-8674(00)80058-6 [pii]
AID - 10.1016/s0092-8674(00)80058-6 [doi]
PST - ppublish
SO  - Cell. 1999 Oct 1;99(1):13-22. doi: 10.1016/s0092-8674(00)80058-6.