PMID- 1052094
OWN - NLM
STAT- MEDLINE
DCOM- 19770331
LR  - 20190827
IS  - 0301-5564 (Print)
IS  - 0301-5564 (Linking)
VI  - 50
IP  - 2
DP  - 1976-1977
TI  - Polyacrylamide gel technique for the histochemical demonstration of soluble
      enzymes.
PG  - 81-9
AB  - Soluble enzymes were immobilized and visualized by polyacrylamide gel slabs,
      impregnated with the incubation medium including auxiliary enzymes. The method
      has several advantages over existing techniques which make use of gel films or a 
      semipermeable membrane. The diffusion of tissue compounds is effectively limited,
      while auxiliary enzymes may be operative. Moreover the viscosity of the medium is
      temperature-independent so that the incubation temperature can be varied. To
      demonstrate the suitability of the method glycerol-3-phosphate dehydrogenase,
      lactate dehydrogenase, glucose-6-phosphate dehydrogenase, hexokinase,
      phosphoglucomutase and aldolase were visulaized in human or rat skeletal muscle. 
      Cytosolic and mitochondrial glycerol-3-phosphate dehydrogenase were both
      visualized in the absence of added NAD+ and menadione. For the visualization of
      ATP producint enzymes, like creatine kinase and pyruvate kinase, the method is
      not suitable.
FAU - Ruitenbeek, W
AU  - Ruitenbeek W
FAU - Scholte, H R
AU  - Scholte HR
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Histochemistry
JT  - Histochemistry
JID - 0411300
RN  - 0 (Enzymes)
RN  - 0 (Gels)
RN  - EC 1.1.- (Glycerolphosphate Dehydrogenase)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 4.1.2.13 (Fructose-Bisphosphate Aldolase)
RN  - EC 5.4.2.2 (Phosphoglucomutase)
SB  - IM
MH  - Animals
MH  - Cytosol/enzymology
MH  - Enzymes/*analysis
MH  - Fructose-Bisphosphate Aldolase/analysis
MH  - Gels
MH  - Glucosephosphate Dehydrogenase/analysis
MH  - Glycerolphosphate Dehydrogenase/analysis
MH  - Hexokinase/analysis
MH  - Histocytochemistry/*methods
MH  - Humans
MH  - L-Lactate Dehydrogenase/analysis
MH  - Muscles/enzymology
MH  - Phosphoglucomutase/analysis
MH  - Rats
EDAT- 1976/01/01 00:00
MHDA- 1976/01/01 00:01
CRDT- 1976/01/01 00:00
PHST- 1976/01/01 00:00 [pubmed]
PHST- 1976/01/01 00:01 [medline]
PHST- 1976/01/01 00:00 [entrez]
AID - 10.1007/bf00495819 [doi]
PST - ppublish
SO  - Histochemistry. 1976-1977;50(2):81-9. doi: 10.1007/bf00495819.