PMID- 10520227
OWN - NLM
STAT- MEDLINE
DCOM- 19991213
LR  - 20100324
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 19
IP  - 4
DP  - 1999 Oct
TI  - Localization of the human 64kD autoantigen D1 to myofibrils in a subset of
      extraocular muscle fibers.
PG  - 313-22
AB  - PURPOSE: To evaluate the tissue-specific expression pattern of the 64kD human
      autoantigen D1, a tropomodulin-related protein that may be involved in
      thyroid-associated ophthalmopathy. METHODS: Recombinant 64kD human autoantigen D1
      was generated in a bacterial expression system and used to immunize rabbits.
      Specific antibodies were affinity-purified and used for Western blots on normal
      and hyperthyroid rat and rabbit tissue, and immunofluorescence localization on
      cryosections of rat tissue. RESULTS: Anti-64kD human autoantigen D1 antibodies
      recognize specifically a approximately 70kD polypeptide in western blots of
      extraocular muscle, sternothyroid muscle, and smooth muscle. Immunofluorescence
      staining demonstrates that the 64kD human autoantigen D1 localizes to myofibrils 
      in slow fibers from rat extraocular and sternothyroid muscle. The level of this
      protein is not altered in extraocular muscles from hyperthyroid rabbits.
      CONCLUSIONS: The 64kD human autoantigen D1 is expressed in slow fibers of
      extraocular and sternothyroid muscles as a component of myofibrils, and is not
      upregulated in conditions of hyperthyroidism.
FAU - Conley, C A
AU  - Conley CA
AD  - Department of Cell Biology, MB24, The Scripps Research Institute 10550 N. Torrey 
      Pines Rd., CA 92037, La Jolla, USA. cconley@mail.arc.nasa.gov
FAU - Fowler, V M
AU  - Fowler VM
LA  - eng
GR  - GM34225/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Actins)
RN  - 0 (Autoantigens)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (LMOD1 protein, human)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tropomyosin)
RN  - EC 3.6.4.1 (Myosins)
SB  - IM
SB  - S
MH  - Actins/metabolism
MH  - Animals
MH  - Autoantigens/genetics/immunology/*metabolism
MH  - Blotting, Western
MH  - Cytoskeletal Proteins
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Escherichia coli/genetics
MH  - Fluorescent Antibody Technique, Indirect
MH  - Gene Expression
MH  - Graves Disease/*metabolism/pathology
MH  - Humans
MH  - Immunoglobulin G/metabolism
MH  - Microscopy, Fluorescence
MH  - Myofibrils/*metabolism/pathology
MH  - Myosins/metabolism
MH  - Oculomotor Muscles/*metabolism/pathology
MH  - Proteins/genetics/immunology/*metabolism
MH  - Rabbits
MH  - Rats
MH  - Recombinant Proteins/genetics/immunology/metabolism
MH  - Tropomyosin/metabolism
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
PST - ppublish
SO  - Curr Eye Res. 1999 Oct;19(4):313-22.