PMID- 10519414
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20081121
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 59
IP  - 19
DP  - 1999 Oct 1
TI  - Genomic structure, chromosomal mapping, and promoter region analysis of murine
      uridine phosphorylase gene.
PG  - 4997-5001
AB  - Uridine phosphorylase (UPase) plays an important role in the activation of
      5-fluorouracil and in the regulation of tissue and plasma concentration of
      uridine, a potential biochemical modulator of 5-fluorouracil therapy. UPase
      expression is affected by the c-H-ras oncogene and various cytokines through
      unknown mechanisms. To understand its expression and regulation, we cloned the
      murine UPase gene, defined its genomic organization, determined its 5'- and
      3'-end flanking sequences, and evaluated the promoter activity. The UPase gene
      contains nine exons and eight introns, spanning a total of approximately 18.0 kb.
      Its promoter lacks canonical TATA and CCAAT boxes, although a CAATAAAAA TATA-like
      box is seen from -41 to -49. Furthermore, IFN regulatory factor 1, c/v-Myb, and
      p53 binding sites are present in the promoter region, indicating that UPase
      expression may be directly regulated by cytokines and oncogene products. The
      1.2-kb flanking fragment showed promoter activity driving the expression of the
      luciferase gene in various mammalian cells. A TGGGG repeat sequence is seen in
      the 3'-end flanking region. This element is considered to be a potential
      recombination consensus hot spot that may contribute to the encoding of different
      UPase isoforms present in different tissues, both normal and neoplastic.
FAU - Cao, D
AU  - Cao D
AD  - Department of Internal Medicine (Oncology), Yale University School of Medicine,
      New Haven, Connecticut 06520, USA.
FAU - Nimmakayalu, M A
AU  - Nimmakayalu MA
FAU - Wang, F
AU  - Wang F
FAU - Zhang, D
AU  - Zhang D
FAU - Handschumacher, R E
AU  - Handschumacher RE
FAU - Bray-Ward, P
AU  - Bray-Ward P
FAU - Pizzorno, G
AU  - Pizzorno G
LA  - eng
GR  - CA 67035/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA-Binding Proteins)
RN  - EC 2.4.2.3 (Uridine Phosphorylase)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - 5' Untranslated Regions/genetics
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/metabolism
MH  - Exons
MH  - *Gene Expression Regulation, Enzymologic
MH  - Introns
MH  - Karyotyping
MH  - Lymphocytes/enzymology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - *Promoter Regions, Genetic
MH  - Restriction Mapping
MH  - Spleen/enzymology
MH  - Uridine Phosphorylase/*genetics
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1999 Oct 1;59(19):4997-5001.