PMID- 10518938
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 458
IP  - 1
DP  - 1999 Sep 10
TI  - One single mRNA encodes the centrosomal protein CCD41 and the endothelial cell
      protein C receptor (EPCR).
PG  - 77-80
AB  - The cDNA encoding the centrosomal protein CCD41 is identical with the cDNA for
      the endothelial cell protein C receptor. This finding is not due to an artefact, 
      e.g. caused by selection of false positive clones. The segment of the CCD41 cDNA 
      encoding the protein originally termed CCD41 and deletion mutants of it were
      fused with the nucleotide sequence encoding the enhanced green fluorescent
      protein (EGFP). Transfection and expression of the full length construct produces
      a fusion protein mainly located in cell membranes reflecting the receptor-type
      protein. Deletion mutants, e.g. those where the signal sequence is deleted,
      result in fusion proteins which are exclusively incorporated into a small
      perinuclear structure which is the site of the centrosome. This result suggests
      that post-translational modification, namely deletion of the signal sequence, is 
      decisive for the centrosomal location of the resulting centrosomal protein while 
      the unprocessed protein is incorporated into cell membranes.
FAU - Rothbarth, K
AU  - Rothbarth K
AD  - Division Biochemistry of the Cell, German Cancer Research Center, Heidelberg,
      Germany.
FAU - Dabaghian, A R
AU  - Dabaghian AR
FAU - Stammer, H
AU  - Stammer H
FAU - Werner, D
AU  - Werner D
LA  - eng
SI  - GENBANK/X63748
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Antigens, CD)
RN  - 0 (Blood Coagulation Factors)
RN  - 0 (DNA, Complementary)
RN  - 0 (Endothelial Protein C Receptor)
RN  - 0 (Glycoproteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (PROCR protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (activated protein C receptor)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD
MH  - *Blood Coagulation Factors
MH  - Carcinoma, Ehrlich Tumor/metabolism
MH  - Cell Membrane/metabolism
MH  - Cells, Cultured
MH  - Centrosome/metabolism
MH  - DNA, Complementary/genetics
MH  - Endothelial Protein C Receptor
MH  - Gene Expression Regulation
MH  - Glycoproteins/*genetics/metabolism
MH  - Green Fluorescent Proteins
MH  - Luminescent Proteins/metabolism
MH  - Microtubules/metabolism
MH  - Molecular Sequence Data
MH  - Protein Processing, Post-Translational
MH  - RNA, Messenger/genetics/*physiology
MH  - Receptors, Cell Surface/*genetics/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Transfection
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - S0014-5793(99)01074-1 [pii]
AID - 10.1016/s0014-5793(99)01074-1 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Sep 10;458(1):77-80. doi: 10.1016/s0014-5793(99)01074-1.