PMID- 10518933
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 458
IP  - 1
DP  - 1999 Sep 10
TI  - The delta subunit of rod specific cyclic GMP phosphodiesterase, PDE delta,
      interacts with the Arf-like protein Arl3 in a GTP specific manner.
PG  - 55-9
AB  - Recently, we have shown that the delta subunit of the cGMP phosphodiesterase (PDE
      delta) interacts with the retinitis pigmentosa guanine regulator (RPGR). Here,
      using the two-hybrid system, we identify a member of the Arf-like protein family 
      of Ras-related GTP-binding proteins, Arl3, that interacts with PDE delta. The
      interaction was verified by fluorescence spectroscopy and co-immunoprecipitation.
      Arl3 features an unusually low affinity for guanine nucleotides, with a KD of 24 
      nM for GDP and 48 microM for GTP. Fluorescence spectroscopy shows that PDE delta 
      binds and specifically stabilizes the GTP-bound form of Arl3 by strongly
      decreasing the dissociation rate of GTP. Thus, PDE delta is an effector of Arl3
      and could provide a novel nucleotide exchange mechanism by which PDE delta
      stabilizes Arl3 in its active GTP-bound form.
FAU - Linari, M
AU  - Linari M
AD  - Max-Planck-Institut fur Molekulare Physiologie, Abteilung Strukturelle Biologie, 
      Dortmund, Germany.
FAU - Hanzal-Bayer, M
AU  - Hanzal-Bayer M
FAU - Becker, J
AU  - Becker J
LA  - eng
SI  - GENBANK/AF143241
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Eye Proteins)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.1.4.35 (3',5'-Cyclic-GMP Phosphodiesterases)
RN  - EC 3.1.4.35 (Cyclic Nucleotide Phosphodiesterases, Type 6)
RN  - EC 3.1.4.35 (PDE6B protein, human)
RN  - EC 3.1.4.35 (Pde6b protein, mouse)
RN  - EC 3.6.1.2 (Arl3 protein, mouse)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factors)
RN  - EC 3.6.5.2 (ARL3 protein, human)
SB  - IM
MH  - 3',5'-Cyclic-GMP Phosphodiesterases/*metabolism/*physiology
MH  - ADP-Ribosylation Factors/*metabolism
MH  - Animals
MH  - Cell Line
MH  - Cyclic Nucleotide Phosphodiesterases, Type 6
MH  - Eye Proteins/*metabolism/*physiology
MH  - Guanosine Triphosphate/metabolism
MH  - Humans
MH  - Kinetics
MH  - Mice
MH  - Mutagenesis
MH  - Plasmids
MH  - Protein Conformation
MH  - Spectrometry, Fluorescence
MH  - Time Factors
MH  - Transfection
MH  - Two-Hybrid System Techniques
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - S0014-5793(99)01117-5 [pii]
AID - 10.1016/s0014-5793(99)01117-5 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Sep 10;458(1):55-9. doi: 10.1016/s0014-5793(99)01117-5.