PMID- 10518925
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 458
IP  - 1
DP  - 1999 Sep 10
TI  - cDNA cloning, characterization and stable expression of novel human brain
      carboxylesterase.
PG  - 17-22
AB  - The DNA sequence encoding a novel human brain carboxylesterase (CES) has been
      determined. The protein is predicted to have 567 amino acids, including conserved
      motifs, such as GESAGG, GXXXXEFG, and GDHGD which comprise a catalytic triad, and
      the endoplasmic reticulum retention motif (HXEL-COOH) observed in CES families.
      Their gene products exhibited hydrolase activity towards temocapril,
      p-nitrophenyl-acetate and long-chain acyl-CoA. Since the molecular masses of
      these gene products are similar to those that exist in capillary endothelial
      cells of the human brain [Yamamda et al. (1994) Brain Res. 658, 163-167], these
      CES isozymes may function as a blood-brain barrier to protect the central nervous
      system from ester or amide compounds.
FAU - Mori, M
AU  - Mori M
AD  - Laboratory of Biochemical Pharmacology and Toxicology, Faculty of Pharmaceutical 
      Sciences, Chiba University, Japan.
FAU - Hosokawa, M
AU  - Hosokawa M
FAU - Ogasawara, Y
AU  - Ogasawara Y
FAU - Tsukada, E
AU  - Tsukada E
FAU - Chiba, K
AU  - Chiba K
LA  - eng
SI  - GENBANK/AB025026
SI  - GENBANK/AB025028
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - EC 3.1.1.- (Carboxylic Ester Hydrolases)
RN  - EC 3.1.1.1 (Carboxylesterase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/*enzymology
MH  - Carboxylesterase
MH  - Carboxylic Ester Hydrolases/chemistry/*genetics/*metabolism
MH  - Catalysis
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary/*metabolism
MH  - *Gene Expression Regulation, Enzymologic
MH  - Gene Library
MH  - Humans
MH  - Isoenzymes/chemistry
MH  - Kinetics
MH  - Liver/metabolism
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
MH  - Transfection
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - S0014-5793(99)01111-4 [pii]
AID - 10.1016/s0014-5793(99)01111-4 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Sep 10;458(1):17-22. doi: 10.1016/s0014-5793(99)01111-4.