PMID- 10518787
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 265
IP  - 3
DP  - 1999 Nov
TI  - Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70
      subunit of DNA polymerase alpha.
PG  - 936-43
AB  - Budding yeast CDC45 encodes Cdc45p, an essential protein required to trigger
      initiation of DNA replication in late G1 phase. We cloned four and one species of
      the human Cdc45p homolog cDNA, resulting from different splicing patterns, from
      HeLa cell and human placenta cDNA libraries, respectively. A comparison of the
      cDNAs and the genomic sequence showed that the longest encoding a 610-amino acid 
      protein was comprised of 20 exons. One species, which lacks exon 7 and contains
      the shorter of two exons 18, was identical with the previously reported CDC45L
      cDNA and constituted 24 out of 28 clones from HeLa cells. Splicing was different 
      in HeLa cells and TIG-1 cells, a human diploid cell line. Human CDC45 protein was
      found to bind directly in vitro to human minichromosome maintenance 7 protein
      (hMCM7) and to the p70 subunit of DNA polymerase alpha. The data support a thesis
      that human CDC45 acts as a molecular tether to mediate loading of the DNA
      polymerase alpha on to the DNA replication complex through binding to hMCM7.
FAU - Kukimoto, I
AU  - Kukimoto I
AD  - Division of Molecular Genetics, National Institute of Infectious Diseases, Tokyo,
      Japan. ikuki@nih.go.jp
FAU - Igaki, H
AU  - Igaki H
FAU - Kanda, T
AU  - Kanda T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (CDC45 protein, S cerevisiae)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.7.- (DNA Polymerase I)
RN  - EC 3.6.4.12 (MCM7 protein, S cerevisiae)
RN  - EC 3.6.4.12 (MCM7 protein, human)
RN  - EC 3.6.4.12 (Minichromosome Maintenance Complex Component 7)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Cycle Proteins/*metabolism
MH  - Cloning, Molecular
MH  - DNA Polymerase I/chemistry/genetics/*metabolism
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - DNA-Binding Proteins/*metabolism
MH  - Escherichia coli/genetics
MH  - Fungal Proteins/genetics/metabolism
MH  - Gene Expression
MH  - HeLa Cells
MH  - Humans
MH  - In Vitro Techniques
MH  - Minichromosome Maintenance Complex Component 7
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Protein Binding
MH  - Protein Structure, Quaternary
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Homology, Amino Acid
MH  - Viral Proteins/genetics
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - ejb791 [pii]
AID - 10.1046/j.1432-1327.1999.00791.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Nov;265(3):936-43. doi: 10.1046/j.1432-1327.1999.00791.x.