PMID- 10517632
OWN - NLM
STAT- MEDLINE
DCOM- 19991019
LR  - 20190613
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 401
IP  - 6751
DP  - 1999 Sep 23
TI  - Casein kinase I transduces Wnt signals.
PG  - 345-50
AB  - The Wnt signalling cascade is essential for the development of both invertebrates
      and vertebrates, and is altered during tumorigenesis. Although a general
      framework for Wnt signalling has been elucidated, not all of the components have 
      been identified. Here we describe a serine kinase, casein kinase I (CKI), which
      was isolated by expression cloning in Xenopus embryos. CKI reproduces several
      properties of Wnt signals, including generation of complete dorsal axes,
      stabilization of beta-catenin and induction of genes that are direct targets of
      Wnt signals. Dominant-negative forms of CKI and a pharmacological blocker of CKI 
      inhibited Wnt signals in Xenopus. Inhibiting CKI in Caenorhabditis elegans
      generated worms with a mom phenotype, indicative of a loss of Wnt signals. In
      addition, CKI bound to and increased the phosphorylation of dishevelled, a known 
      component of the Wnt pathway. These data indicate that CKI may be a conserved
      component of the Wnt pathway.
FAU - Peters, J M
AU  - Peters JM
AD  - Center for Developmental Biology, Department of Molecular Biology, UT
      Southwestern Medical Center, Dallas, Texas 75235-9133, USA.
      graff02@utsw.swmed.edu
FAU - McKay, R M
AU  - McKay RM
FAU - McKay, J P
AU  - McKay JP
FAU - Graff, J M
AU  - Graff JM
LA  - eng
SI  - GENBANK/AF183394
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (CTNNB1 protein, Xenopus)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Dishevelled Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Isoquinolines)
RN  - 0 (Phosphoproteins)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Wnt Proteins)
RN  - 0 (Xenopus Proteins)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (beta Catenin)
RN  - 120615-25-0 (N-(2-aminoethyl)-5-chloroisoquinoline-8-sulfonamide)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Casein Kinases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Caenorhabditis elegans
MH  - Casein Kinases
MH  - Cloning, Molecular
MH  - Cytoskeletal Proteins/metabolism
MH  - Dishevelled Proteins
MH  - Embryo, Nonmammalian/enzymology/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Epistasis, Genetic
MH  - Isoquinolines/pharmacology
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein Kinase Inhibitors
MH  - Protein Kinases/genetics/*metabolism
MH  - Proto-Oncogene Proteins/*metabolism
MH  - *Signal Transduction
MH  - *Trans-Activators
MH  - Wnt Proteins
MH  - Xenopus
MH  - Xenopus Proteins
MH  - *Zebrafish Proteins
MH  - beta Catenin
EDAT- 1999/10/12 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/10/12 09:00
PHST- 1999/10/12 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/10/12 09:00 [entrez]
AID - 10.1038/43830 [doi]
PST - ppublish
SO  - Nature. 1999 Sep 23;401(6751):345-50. doi: 10.1038/43830.