PMID- 10517541
OWN - NLM
STAT- MEDLINE
DCOM- 19991109
LR  - 20190921
IS  - 1071-5762 (Print)
IS  - 1029-2470 (Linking)
VI  - 31
IP  - 4
DP  - 1999 Oct
TI  - Mouse glutaredoxin - cDNA cloning, high level expression in E. coli and its
      possible implication in redox regulation of the DNA binding activity in
      transcription factor PEBP2.
PG  - 357-65
AB  - We have isolated a cDNA encoding glutaredoxin (GRX) from a mouse splenic cDNA
      library. This cDNA encoded a protein of 107 amino acids with a calculated
      molecular weight of 11.9 kDa. The deduced amino acid sequence of glutaredoxin in 
      mouse was highly homologous with that in other mammals (81-89%), containing a
      putative active sequence of -Cys-Pro-Try-Cys-. Recombinant mouse glutaredoxin
      expressed in E. coli showed glutathione-disulfide oxidoreductase activity with
      beta-hydroxyethyl disulfide as its substrate, whereas mutant glutaredoxin (Cys
      22, Cys 25 to Ser) showed no activity. In electrophoretic mobility shift assay,
      we proved that wild type GRX, not mutant one, recovered the DNA-binding activity 
      of a transcription factor, PEBP2, oxidized by diamide. This showed that GRX may
      be involved in the redox regulation of the DNA-binding activity of PEBP2 as is
      the case with thioredoxin.
FAU - Nakamura, T
AU  - Nakamura T
AD  - Department of Biological Responses, Institute for Virus Research, Kyoto
      University, Japan.
FAU - Ohno, T
AU  - Ohno T
FAU - Hirota, K
AU  - Hirota K
FAU - Nishiyama, A
AU  - Nishiyama A
FAU - Nakamura, H
AU  - Nakamura H
FAU - Wada, H
AU  - Wada H
FAU - Yodoi, J
AU  - Yodoi J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glutaredoxins)
RN  - 0 (Proteins)
RN  - 0 (Transcription Factor AP-2)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
RN  - EC 1.- (Oxidoreductases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA/genetics/*metabolism
MH  - DNA, Complementary/*genetics/isolation & purification
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Escherichia coli
MH  - Glutaredoxins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - *Oxidoreductases
MH  - Proteins/*genetics/*metabolism
MH  - Transcription Factor AP-2
MH  - Transcription Factors/genetics/*metabolism
RF  - 43
EDAT- 1999/10/12 00:00
MHDA- 1999/10/12 00:01
CRDT- 1999/10/12 00:00
PHST- 1999/10/12 00:00 [pubmed]
PHST- 1999/10/12 00:01 [medline]
PHST- 1999/10/12 00:00 [entrez]
AID - 10.1080/10715769900300931 [doi]
PST - ppublish
SO  - Free Radic Res. 1999 Oct;31(4):357-65. doi: 10.1080/10715769900300931.