PMID- 10516138
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20181002
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 277
IP  - 4
DP  - 1999 Oct
TI  - Regulation of fatty acid oxidation of the heart by MCD and ACC during contractile
      stimulation.
PG  - E772-7
LID - 10.1152/ajpendo.1999.277.4.E772 [doi]
AB  - We tested the hypothesis that the level of malonyl-CoA, as well as the
      corresponding rate of total fatty acid oxidation of the heart, is regulated by
      the opposing actions of acetyl-CoA carboxylase (ACC) and malonyl-CoA
      decarboxylase (MCD). We used isolated working rat hearts perfused under
      physiological conditions. MCD in heart homogenates was measured specifically by
      (14)CO(2) production from [3-(14)C]malonyl-CoA, and ACC was measured specifically
      based on the portion of total carboxylase that is citrate sensitive. Increased
      heart work (1 microM epinephrine + 40% increase in afterload) elicited a 40%
      increase in total beta-oxidation of exogenous plus endogenous lipids, accompanied
      by a 33% decrease in malonyl-CoA. The basal activity and citrate sensitivity of
      ACC (reflecting its phosphorylation state) and citrate content were unchanged.
      AMP levels were also unchanged. MCD activity, when measured at a subsaturating
      concentration of malonyl-CoA (50 microM), was increased by 55%. We conclude that 
      physiological increments in AMP during the work transition are insufficient to
      promote ACC phosphorylation by AMP-stimulated protein kinase. Rather, increased
      fatty acid oxidation results from increased malonyl-CoA degradation by MCD.
FAU - Goodwin, G W
AU  - Goodwin GW
AD  - Division of Cardiology, Department of Internal Medicine, University of
      Texas-Houston Medical School, Houston, Texas 77030, USA.
      ggoodwin@heart.med.uth.tmc.edu
FAU - Taegtmeyer, H
AU  - Taegtmeyer H
LA  - eng
GR  - R01-43133/PHS HHS/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Fatty Acids)
RN  - 524-14-1 (Malonyl Coenzyme A)
RN  - EC 4.1.1.- (Carboxy-Lyases)
RN  - EC 4.1.1.9 (malonyl-CoA decarboxylase)
RN  - EC 6.4.1.2 (Acetyl-CoA Carboxylase)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - Acetyl-CoA Carboxylase/*pharmacology
MH  - Animals
MH  - Carboxy-Lyases/*pharmacology
MH  - Epinephrine/pharmacology
MH  - Fatty Acids/*metabolism
MH  - Heart/drug effects
MH  - In Vitro Techniques
MH  - Malonyl Coenzyme A/metabolism
MH  - Myocardial Contraction/*physiology
MH  - Myocardium/*metabolism
MH  - Oxidation-Reduction
MH  - Rats
EDAT- 1999/10/12 00:00
MHDA- 1999/10/12 00:01
CRDT- 1999/10/12 00:00
PHST- 1999/10/12 00:00 [pubmed]
PHST- 1999/10/12 00:01 [medline]
PHST- 1999/10/12 00:00 [entrez]
AID - 10.1152/ajpendo.1999.277.4.E772 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Oct;277(4):E772-7. doi: 10.1152/ajpendo.1999.277.4.E772.