PMID- 10516074
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 73
IP  - 11
DP  - 1999 Nov
TI  - Molecular characterization of a porcine enteric calicivirus genetically related
      to Sapporo-like human caliciviruses.
PG  - 9625-31
AB  - Porcine enteric calicivirus (PEC) is associated with diarrhea in pigs, and to
      date it is the only cultivable enteric calicivirus (tissue culture-adapted [TC]
      PEC/Cowden). Based on sequence analysis of cDNA clones and reverse
      transcription-PCR products, TC PEC/Cowden has an RNA genome of 7,320 bp,
      excluding its 3' poly(A)(+) tail. The genome is organized in two open reading
      frames (ORFs), similar to the organizations of the human Sapporo-like viruses
      (SLVs) and the lagoviruses. ORF1 encodes the polyprotein that is fused to and
      contiguous with the capsid protein. ORF2 at the 3' end encodes a small basic
      protein of 164 amino acids. Among caliciviruses, PEC has the highest amino acid
      sequence identities in the putative RNA polymerase (66%), 2C helicase (49.6%),
      3C-like protease (43.7%), and capsid (39%) regions with the SLVs, indicating that
      PEC is genetically most closely related to the SLVs. The complete RNA genome of
      wild-type (WT) PEC/Cowden was also sequenced. Sequence comparisons revealed that 
      the WT and TC PEC/Cowden have 100% nucleotide sequence identities in the 5'
      terminus, 2C helicase, ORF2, and the 3' nontranslated region. TC PEC/Cowden has
      one silent mutation in its protease, two amino acid changes and a silent mutation
      in its RNA polymerase, and five nucleotide substitutions in its capsid that
      result in one distant and three clustered amino acid changes and a silent
      mutation. These substitutions may be associated with adaptation of TC PEC/Cowden 
      to cell culture. The cultivable PEC should be a useful model for studies of the
      pathogenesis, replication, and possible rescue of uncultivable human enteric
      caliciviruses.
FAU - Guo, M
AU  - Guo M
AD  - Food Animal Health Research Program, Department of Veterinary Preventive
      Medicine, Ohio Agricultural Research and Development Center, The Ohio State
      University, Wooster, Ohio 44691, USA.
FAU - Chang, K O
AU  - Chang KO
FAU - Hardy, M E
AU  - Hardy ME
FAU - Zhang, Q
AU  - Zhang Q
FAU - Parwani, A V
AU  - Parwani AV
FAU - Saif, L J
AU  - Saif LJ
LA  - eng
SI  - GENBANK/AF182760
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Caliciviridae/classification/*genetics/isolation & purification
MH  - Caliciviridae Infections/*veterinary/virology
MH  - DNA, Complementary/genetics
MH  - Diarrhea/veterinary/virology
MH  - Genome, Viral
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sequence Analysis, RNA
MH  - Swine
MH  - Swine Diseases/*virology
PMC - PMC113000
EDAT- 1999/10/09 00:00
MHDA- 1999/10/09 00:01
CRDT- 1999/10/09 00:00
PHST- 1999/10/09 00:00 [pubmed]
PHST- 1999/10/09 00:01 [medline]
PHST- 1999/10/09 00:00 [entrez]
AID - 10.1128/JVI.73.11.9625-9631.1999 [doi]
PST - ppublish
SO  - J Virol. 1999 Nov;73(11):9625-31. doi: 10.1128/JVI.73.11.9625-9631.1999.