PMID- 10516026
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 73
IP  - 11
DP  - 1999 Nov
TI  - Genome-wide screening, cloning, chromosomal assignment, and expression of
      full-length human endogenous retrovirus type K.
PG  - 9187-95
AB  - The human genome harbors 25 to 50 proviral copies of the endogenous retrovirus
      type K (HERV-K), some of which code for the characteristic retroviral proteins
      Gag, Pol, and Env. For a genome-wide cloning approach of full-length and intact
      HERV-K proviruses, a human P1 gene library was screened with a gag-specific
      probe. Both HERV-K type 1 and 2 clones were isolated. Sixteen HERV-K type 2
      proviral genomes were characterized by direct coupled in vitro transcription-in
      vitro translation assays to analyze the coding potential of isolated gag, pol,
      and env amplicons from individual P1 clones. After determination of long terminal
      repeat (LTR) sequences and adjacent chromosomal integration sites by inverse PCR 
      techniques, two HERV-K type 2 proviruses displaying long retroviral open reading 
      frames (ORFs) were assigned to chromosomes 7 (C7) and 19 (C19) by using a
      human-rodent monochromosomal cell hybrid mapping panel. HERV-K(C7) shows an
      altered (YIDD-to-CIDD) motif in the reverse transcriptase domain. HERV-K(C19) is 
      truncated in the 5' LTR and harbors a defective protease gene due to a point
      mutation. Direct amplification of proviral structures from single chromosomes by 
      using chromosomal flanking primers was performed by long PCR for HERV-K(C7) and
      HERV-K(C19) and for type 1 proviruses HERV-K10 and HERV-K18 from chromosomes 5
      and 1, respectively. HERV-K18, in contrast to HERV-K10, bears no intact gag ORF
      and shows close homology to HERV-K/IDDMK(1,2)22. In transfection experiments,
      HERV-K(C7) and HERV-K cDNA-based expression vectors yielded the proteins Gag and 
      cORF whereas HERV-K10 vectors yielded Gag alone. The data suggest that the human 
      genome does not contain an entire, intact proviral copy of HERV-K.
FAU - Tonjes, R R
AU  - Tonjes RR
AD  - Paul-Ehrlich-Institut, D-63225 Langen, Germany.
FAU - Czauderna, F
AU  - Czauderna F
FAU - Kurth, R
AU  - Kurth R
LA  - eng
SI  - GENBANK/Y17832
SI  - GENBANK/Y17833
SI  - GENBANK/Y17834
SI  - GENBANK/Y18890
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 19
MH  - *Chromosomes, Human, Pair 7
MH  - *Cloning, Molecular
MH  - Dogs
MH  - Endogenous Retroviruses/*genetics/physiology
MH  - Gene Expression
MH  - Gene Library
MH  - Genetic Vectors
MH  - *Genome, Viral
MH  - Humans
MH  - Molecular Sequence Data
MH  - Open Reading Frames/*genetics
MH  - Polymerase Chain Reaction/methods
MH  - Protein Biosynthesis
MH  - Proviruses
MH  - Sequence Analysis, DNA
MH  - Terminal Repeat Sequences/genetics
MH  - Transcription, Genetic
PMC - PMC112952
EDAT- 1999/10/09 00:00
MHDA- 1999/10/09 00:01
CRDT- 1999/10/09 00:00
PHST- 1999/10/09 00:00 [pubmed]
PHST- 1999/10/09 00:01 [medline]
PHST- 1999/10/09 00:00 [entrez]
AID - 10.1128/JVI.73.11.9187-9195.1999 [doi]
PST - ppublish
SO  - J Virol. 1999 Nov;73(11):9187-95. doi: 10.1128/JVI.73.11.9187-9195.1999.