PMID- 10514455
OWN - NLM
STAT- MEDLINE
DCOM- 19991119
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 42
DP  - 1999 Oct 15
TI  - Phosphatidic acid synthesis in mitochondria. Topography of formation and
      transmembrane migration.
PG  - 29786-90
AB  - The topography of formation and migration of phosphatidic acid (PA) in the
      transverse plane of rat liver mitochondrial outer membrane (MOM) were
      investigated. Isolated mitochondria and microsomes, incubated with sn-glycerol
      3-phosphate and an immobilized substrate palmitoyl-CoA-agarose, synthesized both 
      lyso-PA and PA. The mitochondrial and microsomal acylation of glycerophosphate
      with palmitoyl-CoA-agarose was 80-100% of the values obtained in the presence of 
      free palmitoyl-CoA. In another series of experiments, both free polymyxin B and
      polymyxin B-agarose stimulated mitochondrial glycerophosphate acyltransferase
      activity approximately 2-fold. When PA loaded mitochondria were treated with
      liver fatty acid binding protein, a fifth of the phospholipid left the
      mitochondria. The amount of exportable PA reduced with the increase in the time
      of incubation. In another approach, PA-loaded mitochondria were treated with
      phospholipase A(2). The amount of phospholipase A(2)-sensitive PA reduced when
      the incubation time was increased. Taken together, the results suggest that
      lysophosphatidic acid (LPA) and PA are synthesized on the outer surface of the
      MOM and that PA moves to the inner membrane presumably for cardiolipin formation.
FAU - Chakraborty, T R
AU  - Chakraborty TR
AD  - Department of Biological Sciences, St. John's University, Jamaica, New York
      11439, USA.
FAU - Vancura, A
AU  - Vancura A
FAU - Balija, V S
AU  - Balija VS
FAU - Haldar, D
AU  - Haldar D
LA  - eng
GR  - GM-57643/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Fabp7 protein, rat)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Myelin P2 Protein)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phosphatidic Acids)
RN  - EC 2.3.1.15 (Glycerol-3-Phosphate O-Acyltransferase)
SB  - IM
MH  - Acylation
MH  - Animals
MH  - Biological Transport
MH  - Carrier Proteins/metabolism
MH  - Cell Membrane/metabolism
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Glycerol-3-Phosphate O-Acyltransferase/metabolism
MH  - Male
MH  - Microsomes, Liver/enzymology
MH  - Mitochondria, Liver/*metabolism
MH  - Myelin P2 Protein/metabolism
MH  - *Neoplasm Proteins
MH  - *Nerve Tissue Proteins
MH  - Phosphatidic Acids/*biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1999/10/09 00:00
MHDA- 1999/10/09 00:01
CRDT- 1999/10/09 00:00
PHST- 1999/10/09 00:00 [pubmed]
PHST- 1999/10/09 00:01 [medline]
PHST- 1999/10/09 00:00 [entrez]
AID - 10.1074/jbc.274.42.29786 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 15;274(42):29786-90. doi: 10.1074/jbc.274.42.29786.