PMID- 10514424
OWN - NLM
STAT- MEDLINE
DCOM- 19991119
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 42
DP  - 1999 Oct 15
TI  - beta-TrCP mediates the signal-induced ubiquitination of IkappaBbeta.
PG  - 29591-4
AB  - We have examined the role of beta-TrCP (beta-transducin repeat-containing
      protein) in the ubiquitination and degradation of IkappaBbeta, one of the two
      major IkappaB isoforms in mammalian cells. We demonstrate that beta-TrCP
      interacts specifically with IkappaBbeta, and such interaction is dependent on
      prior phosphorylation of IkappaBbeta on serines 19 and 23. Interaction with
      beta-TrCP is also necessary for ubiquitination of IkappaBbeta upon stimulation of
      cells, and deletion of the F-box in beta-TrCP abolishes its ability to
      ubiquitinate IkappaBbeta. Therefore, these results indicate that beta-TrCP plays 
      a critical role in the activation of NF-kappaB by assembling the ubiquitin ligase
      complex for both phosphorylated IkappaBalpha and IkappaBbeta.
FAU - Wu, C
AU  - Wu C
AD  - Section of Immunobiology, Department of Molecular Biophysics, Howard Hughes
      Medical Institute, Yale University School of Medicine, New Haven, Connecticut
      06520, USA.
FAU - Ghosh, S
AU  - Ghosh S
LA  - eng
GR  - R01 AI33443/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (BTRC protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (I kappa B beta protein)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Ubiquitins)
RN  - 0 (beta-Transducin Repeat-Containing Proteins)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Humans
MH  - *I-kappa B Proteins
MH  - Phosphorylation
MH  - Protein Binding
MH  - Ubiquitins/*metabolism
MH  - beta-Transducin Repeat-Containing Proteins
EDAT- 1999/10/09 00:00
MHDA- 1999/10/09 00:01
CRDT- 1999/10/09 00:00
PHST- 1999/10/09 00:00 [pubmed]
PHST- 1999/10/09 00:01 [medline]
PHST- 1999/10/09 00:00 [entrez]
AID - 10.1074/jbc.274.42.29591 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 15;274(42):29591-4. doi: 10.1074/jbc.274.42.29591.