PMID- 10512872
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 10
IP  - 10
DP  - 1999 Oct
TI  - GERp95, a membrane-associated protein that belongs to a family of proteins
      involved in stem cell differentiation.
PG  - 3357-72
AB  - A panel of mAbs was elicited against intracellular membrane fractions from rat
      pancreas. One of the antibodies reacted with a 95-kDa protein that localizes
      primarily to the Golgi complex or the endoplasmic reticulum (ER), depending on
      cell type. The corresponding cDNA was cloned and sequenced and found to encode a 
      protein of 97.6 kDa that we call GERp95 (Golgi ER protein 95 kDa). The protein
      copurifies with intracellular membranes but does not contain hydrophobic regions 
      that could function as signal peptides or transmembrane domains. Biochemical
      analysis suggests that GERp95 is a cytoplasmically exposed peripheral membrane
      protein that exists in a protease-resistant complex. GERp95 belongs to a family
      of highly conserved proteins in metazoans and Schizosaccharomyces pombe. It has
      recently been determined that plant and Drosophila homologues of GERp95 are
      important for controlling the differentiation of stem cells (Bohmert et al.,
      1998; Cox et al., 1998; Moussian et al., 1998). In Caenorhabditis elegans, there 
      are at least 20 members of this protein family. To this end, we have used RNA
      interference to show that the GERp95 orthologue in C. elegans is important for
      maturation of germ-line stem cells in the gonad. GERp95 and related proteins are 
      an emerging new family of proteins that have important roles in metazoan
      development. The present study suggests that these proteins may exert their
      effects on cell differentiation from the level of intracellular membranes.
FAU - Cikaluk, D E
AU  - Cikaluk DE
AD  - Department of Cell Biology, University of Alberta, Edmonton, AB, T6G 2H7, Canada.
FAU - Tahbaz, N
AU  - Tahbaz N
FAU - Hendricks, L C
AU  - Hendricks LC
FAU - DiMattia, G E
AU  - DiMattia GE
FAU - Hansen, D
AU  - Hansen D
FAU - Pilgrim, D
AU  - Pilgrim D
FAU - Hobman, T C
AU  - Hobman TC
LA  - eng
SI  - GENBANK/AF195534
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Ago2 protein, rat)
RN  - 0 (Argonaute Proteins)
RN  - 0 (Eukaryotic Initiation Factor-2)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Argonaute Proteins
MH  - COS Cells
MH  - Caenorhabditis elegans/metabolism
MH  - *Cell Differentiation
MH  - Cloning, Molecular
MH  - Endoplasmic Reticulum/metabolism
MH  - Eukaryotic Initiation Factor-2
MH  - Fluorescent Antibody Technique
MH  - Germ Cells/metabolism
MH  - Golgi Apparatus/metabolism
MH  - Intracellular Membranes/chemistry
MH  - Membrane Proteins/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Pancreas/*chemistry
MH  - RNA, Messenger/metabolism
MH  - Recombinant Proteins
MH  - Reproduction
MH  - Sequence Alignment
MH  - Stem Cells/cytology/*metabolism
MH  - Transfection
PMC - PMC25603
EDAT- 1999/10/08 00:00
MHDA- 1999/10/08 00:01
CRDT- 1999/10/08 00:00
PHST- 1999/10/08 00:00 [pubmed]
PHST- 1999/10/08 00:01 [medline]
PHST- 1999/10/08 00:00 [entrez]
AID - 10.1091/mbc.10.10.3357 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1999 Oct;10(10):3357-72. doi: 10.1091/mbc.10.10.3357.