PMID- 10512864
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 10
IP  - 10
DP  - 1999 Oct
TI  - Apoptosis induced by the nuclear death domain protein p84N5 is inhibited by
      association with Rb protein.
PG  - 3251-61
AB  - Rb protein inhibits both cell cycle progression and apoptosis. Interaction of
      specific cellular proteins, including E2F1, with Rb C-terminal domains mediates
      cell cycle regulation. In contrast, the nuclear N5 protein associates with an Rb 
      N-terminal domain with unknown function. The N5 protein contains a region of
      sequence similarity to the death domain of proteins involved in apoptotic
      signaling. We demonstrate here that forced N5 expression potently induces
      apoptosis in several tumor cell lines. Mutation of conserved residues within the 
      death domain homology compromise N5-induced apoptosis, suggesting that it is
      required for normal function. Endogenous N5 protein is specifically altered in
      apoptotic cells treated with ionizing radiation. Furthermore, dominant
      interfering death domain mutants compromise cellular responses to ionizing
      radiation. Finally, physical association with Rb protein inhibits N5-induced
      apoptosis. We propose that N5 protein plays a role in the regulation of apoptosis
      and that Rb directly coordinates cell proliferation and apoptosis by binding
      specific proteins involved in each process through distinct protein binding
      domains.
FAU - Doostzadeh-Cizeron, J
AU  - Doostzadeh-Cizeron J
AD  - Department of Cancer Biology, University of Texas M. D. Anderson Cancer Center,
      Houston, Texas 77030, USA.
FAU - Evans, R
AU  - Evans R
FAU - Yin, S
AU  - Yin S
FAU - Goodrich, D W
AU  - Goodrich DW
LA  - eng
GR  - P30 CA016672/CA/NCI NIH HHS/United States
GR  - R01 CA070292/CA/NCI NIH HHS/United States
GR  - CA-16672/CA/NCI NIH HHS/United States
GR  - CA-70292-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (THOC1 protein, human)
RN  - H88EPA0A3N (Staurosporine)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Apoptosis
MH  - Cell Cycle
MH  - *Cell Cycle Proteins
MH  - Cell Line
MH  - DNA Fragmentation
MH  - Fluorescent Antibody Technique
MH  - Gamma Rays
MH  - Humans
MH  - Microinjections
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Nuclear Proteins/chemistry/*genetics/*metabolism
MH  - Protein Binding
MH  - Recombinant Fusion Proteins
MH  - Retinoblastoma Protein/*metabolism
MH  - Staurosporine/pharmacology
MH  - Transfection
PMC - PMC25587
EDAT- 1999/10/08 00:00
MHDA- 1999/10/08 00:01
CRDT- 1999/10/08 00:00
PHST- 1999/10/08 00:00 [pubmed]
PHST- 1999/10/08 00:01 [medline]
PHST- 1999/10/08 00:00 [entrez]
AID - 10.1091/mbc.10.10.3251 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1999 Oct;10(10):3251-61. doi: 10.1091/mbc.10.10.3251.