PMID- 10512748
OWN - NLM
STAT- MEDLINE
DCOM- 19991123
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 263
IP  - 3
DP  - 1999 Oct 5
TI  - Genomic organization and structure of the 3' region of human MUC3: alternative
      splicing predicts membrane-bound and soluble forms of the mucin.
PG  - 728-36
AB  - The MUC3 gene encodes a large, glycosylated mucin produced by intestinal
      epithelial cells to form a protective barrier against the external environment.
      Recently published cDNA sequences for the carboxyl-terminal region of MUC3
      polypeptide indicated that rodent Muc3 possesses two epidermal growth factor
      (EGF)-like domains, and putative transmembrane and cytoplasmic domains, whereas
      the sequence of human MUC3 predicted termination after the first EGF-like domain.
      Here we describe the complete genomic sequence encompassing the carboxyl terminal
      region of human MUC3, revealing the boundaries of 11 exons. RT-PCR and cDNA
      library cloning experiments indicate that the gene is alternatively spliced,
      yielding a major membrane-bound form as well as multiple soluble forms. Thus,
      this work indicates that both membrane-bound and soluble MUC3 mucin proteins are 
      produced by alternative splicing of a single gene. A potentially important
      polymorphism involving a Tyr residue with a proposed role in signalling is
      described as well.
CI  - Copyright 1999 Academic Press.
FAU - Crawley, S C
AU  - Crawley SC
AD  - Department of Medicine, University of California, San Francisco, California
      94143, USA.
FAU - Gum, J R Jr
AU  - Gum JR Jr
FAU - Hicks, J W
AU  - Hicks JW
FAU - Pratt, W S
AU  - Pratt WS
FAU - Aubert, J P
AU  - Aubert JP
FAU - Swallow, D M
AU  - Swallow DM
FAU - Kim, Y S
AU  - Kim YS
LA  - eng
SI  - GENBANK/AF113616
GR  - CA24321/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Muc3 protein, mouse)
RN  - 0 (Muc3 protein, rat)
RN  - 0 (Mucin-3)
RN  - 0 (Mucins)
RN  - 0 (Neoplasm Proteins)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - 3' Untranslated Regions/*genetics
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Membrane/metabolism
MH  - Conserved Sequence
MH  - Cytoplasm/metabolism
MH  - Epidermal Growth Factor/chemistry
MH  - Exons
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mucin-3
MH  - Mucins/*chemistry/*genetics/metabolism
MH  - Neoplasm Proteins/chemistry/genetics/metabolism
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
EDAT- 1999/10/08 09:00
MHDA- 2001/03/28 10:01
CRDT- 1999/10/08 09:00
PHST- 1999/10/08 09:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/10/08 09:00 [entrez]
AID - 10.1006/bbrc.1999.1466 [doi]
AID - S0006-291X(99)91466-3 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Oct 5;263(3):728-36. doi:
      10.1006/bbrc.1999.1466.