PMID- 10512684
OWN - NLM
STAT- MEDLINE
DCOM- 19991109
LR  - 20191210
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 61
IP  - 1
DP  - 1999 Oct 1
TI  - Detection and cloning of an X-linked locus associated with a NotI site that is
      not methylated on mouse inactivated X chromosome by the RLGS-M method.
PG  - 92-100
AB  - In looking for genes that escape X chromosome inactivation, we scanned the
      methylation status of genomic DNA from XX, X0, and XY mice using the method of
      restriction landmark genomic scanning using methylation-sensitive endonuclease.
      We detected and cloned a candidate locus and identified the Orf1 gene. Orf1 has
      sequence similarities to the B2 repetitive element and human CXORF4 (formerly
      called EXLM1), which escapes X inactivation. The B2 element spans the 3' terminus
      of the ORF and the 3' UTR of Orf1. The Orf1 gene encompasses 18.5 kb of genomic
      DNA including 11 exons and 10 introns. Taking advantage of genomic polymorphisms 
      present between MSM and C3H/He, we showed that murine Orf1 is mapped to the
      proximal region of the X chromosome. Despite the unmethylation of the NotI site, 
      Orf1 is subject to X inactivation.
CI  - Copyright 1999 Academic Press.
FAU - Takada, S
AU  - Takada S
AD  - Graduate School of Environmental Earth Science, Hokkaido University, North 10
      West 5 Kita-ku, Sapporo, 060-0810, Japan.
FAU - Kamiya, M
AU  - Kamiya M
FAU - Arima, T
AU  - Arima T
FAU - Kagebayashi, H
AU  - Kagebayashi H
FAU - Shibata, H
AU  - Shibata H
FAU - Muramatsu, M
AU  - Muramatsu M
FAU - Chapman, V M
AU  - Chapman VM
FAU - Wake, N
AU  - Wake N
FAU - Hayashizaki, Y
AU  - Hayashizaki Y
FAU - Takagi, N
AU  - Takagi N
LA  - eng
SI  - GENBANK/AB019028
SI  - GENBANK/AB019029
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Med14 protein, mouse)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.1.21.4 (GCGGCCGC-specific type II deoxyribonucleases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - CpG Islands
MH  - DNA Methylation
MH  - Deoxyribonucleases, Type II Site-Specific
MH  - *Dosage Compensation, Genetic
MH  - Female
MH  - Gene Expression
MH  - Genetic Linkage
MH  - Humans
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Proteins/*genetics
MH  - RNA, Messenger/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Transcription Factors
MH  - *X Chromosome
EDAT- 1999/10/08 00:00
MHDA- 1999/10/08 00:01
CRDT- 1999/10/08 00:00
PHST- 1999/10/08 00:00 [pubmed]
PHST- 1999/10/08 00:01 [medline]
PHST- 1999/10/08 00:00 [entrez]
AID - 10.1006/geno.1999.5944 [doi]
AID - S0888-7543(99)95944-X [pii]
PST - ppublish
SO  - Genomics. 1999 Oct 1;61(1):92-100. doi: 10.1006/geno.1999.5944.