PMID- 10510470
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20191210
IS  - 1350-9047 (Print)
IS  - 1350-9047 (Linking)
VI  - 6
IP  - 9
DP  - 1999 Sep
TI  - WW domain-containing FBP-30 is regulated by p53.
PG  - 883-9
AB  - A subtractive cloning approach was used to clone genes transcriptionally induced 
      in thymocytes undergoing programmed cell death after gamma-irradiation. We thus
      identified about 60 upregulated genes. One of these genes encodes a WW domain
      previously briefly reported by others, which defines this gene as FBP-30. cDNA
      sequencing showed FBP-30 to be remarkably conserved in mammals. FBP-30
      expression, essentially restricted to T cells, was regulated by p53 since it
      increased (1) after gamma-irradiation in wild-type but not in p53-/- thymocytes
      and (2) in cells transfected with a conditional temperature-sensitive p53 mutant,
      less than 1 h after shifting to permissive temperature. Upregulation of FBP-30
      expression thus depends upon p53 protein expression and correlates with cell
      death induction in these systems. While the kinetics of its induction are rapid, 
      the observed increased expression of FBP-30 in the presence of protein synthesis 
      inhibitors suggests that FBP-30 gene expression is indirectly regulated by p53,
      through downregulation of a labile inhibitor of FBP-30 expression.
FAU - Depraetere, V
AU  - Depraetere V
AD  - Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288 Marseille
      Cedex 9, France.
FAU - Golstein, P
AU  - Golstein P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Death Differ
JT  - Cell death and differentiation
JID - 9437445
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (FNBP4 protein, human)
RN  - 0 (Fnbp4 protein, mouse)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - *Carrier Proteins
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - DNA, Complementary
MH  - Female
MH  - Gamma Rays
MH  - *Gene Expression Regulation/radiation effects
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Protein Structure, Tertiary
MH  - Proteins/*genetics
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Thymus Gland/cytology
MH  - Tumor Suppressor Protein p53/*metabolism
EDAT- 1999/10/08 00:00
MHDA- 1999/10/08 00:01
CRDT- 1999/10/08 00:00
PHST- 1999/10/08 00:00 [pubmed]
PHST- 1999/10/08 00:01 [medline]
PHST- 1999/10/08 00:00 [entrez]
AID - 10.1038/sj.cdd.4400564 [doi]
PST - ppublish
SO  - Cell Death Differ. 1999 Sep;6(9):883-9. doi: 10.1038/sj.cdd.4400564.