PMID- 10510269
OWN - NLM
STAT- MEDLINE
DCOM- 20000405
LR  - 20190516
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 32
IP  - 10
DP  - 1999 Oct
TI  - Functional characterization and localization of AQP3 in the human colon.
PG  - 1303-13
AB  - Water channels or aquaporins (AQPs) have been identified in a large variety of
      tissues. Nevertheless, their role in the human gastrointestinal tract, where
      their action is essential for the reabsorption and secretion of water and
      electrolytes, is still unclear. The purpose of the present study was to
      investigate the structure and function of water channels expressed in the human
      colon. A cDNA fragment of about 420 bp with a 98% identity to human AQP3 was
      amplified from human stomach, small intestine and colon by reverse transcription 
      polymerase chain reaction (RT-PCR) and a transcript of 2.2 kb was expressed more 
      abundantly in colon than in jejunum, ileum and stomach as indicated by Northern
      blots. Expression of mRNA from the colon of adults and children but not from
      other gastrointestinal regions in Xenopus oocytes enhanced the osmotic water
      permeability, and the urea and glycerol transport in a manner sensitive to an
      antisense AQP3 oligonucleotide, indicating the presence of functional AQP3.
      Immunocytochemistry and immunofluorescence studies in human colon revealed that
      the AQP3 protein is restricted to the villus epithelial cells. The immunostaining
      within these cells was more intense in the apical than in the basolateral
      membranes. The presence of AQP3 in villus epithelial cells suggests that AQP3 is 
      implicated in water absorption across human colonic surface cells.
FAU - Silberstein, C
AU  - Silberstein C
AD  - Laboratorio de Fisiopatogenia, Departamento de Fisiologia, Facultad de Medicina, 
      Universidad Nacional de Buenos Aires, Buenos Aires, Argentina.
FAU - Kierbel, A
AU  - Kierbel A
FAU - Amodeo, G
AU  - Amodeo G
FAU - Zotta, E
AU  - Zotta E
FAU - Bigi, F
AU  - Bigi F
FAU - Berkowski, D
AU  - Berkowski D
FAU - Ibarra, C
AU  - Ibarra C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (AQP3 protein, human)
RN  - 0 (Aquaporins)
RN  - 0 (RNA, Messenger)
RN  - 158801-98-0 (Aquaporin 3)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - Aquaporin 3
MH  - Aquaporins/chemistry/genetics/*physiology
MH  - Blotting, Northern
MH  - Cell Membrane Permeability
MH  - Child
MH  - Child, Preschool
MH  - Colon/*chemistry
MH  - Epithelial Cells/chemistry
MH  - Fluoroimmunoassay
MH  - Humans
MH  - Immunohistochemistry
MH  - Intestinal Absorption/*physiology
MH  - Molecular Sequence Data
MH  - Oocytes
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Xenopus laevis
EDAT- 1999/10/06 09:00
MHDA- 2001/07/04 10:01
CRDT- 1999/10/06 09:00
PHST- 1999/10/06 09:00 [pubmed]
PHST- 2001/07/04 10:01 [medline]
PHST- 1999/10/06 09:00 [entrez]
AID - S0100-879X(99)03201018 [pii]
AID - 10.1590/s0100-879x1999001000018 [doi]
PST - ppublish
SO  - Braz J Med Biol Res. 1999 Oct;32(10):1303-13. doi:
      10.1590/s0100-879x1999001000018.