PMID- 10509717
OWN - NLM
STAT- MEDLINE
DCOM- 19991101
LR  - 20190910
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 63
IP  - 1-2
DP  - 1999 Sep
TI  - Comparative sequence analyses of sixteen reptilian paramyxoviruses.
PG  - 65-74
AB  - Viral genomic RNA of Fer-de-Lance virus (FDLV), a paramyxovirus highly pathogenic
      for reptiles, was reverse transcribed and cloned. Plasmids with significant
      sequence similarities to the hemagglutinin-neuraminidase (HN) and polymerase (L) 
      genes of mammalian paramyxoviruses were identified by BLAST search. Partial
      sequences of the FDLV genes were used to design primers for amplification by
      nested polymerase chain reaction (PCR) and sequencing of 518-bp L gene and 352-bp
      HN gene fragments from a collection of 15 previously uncharacterized reptilian
      paramyxoviruses. Phylogenetic analyses of the partial L and HN sequences produced
      similar trees in which there were two distinct subgroups of isolates that were
      supported with maximum bootstrap values, and several intermediate isolates.
      Within each subgroup the nucleotide divergence values were less than 2.5%, while 
      the divergence between the two subgroups was 20-22%. This indicated that the two 
      subgroups represent distinct virus species containing multiple virus strains. The
      five intermediate isolates had nucleotide divergence values of 11-20% and may
      represent additional distinct species. In addition to establishing diversity
      among reptilian paramyxoviruses, the phylogenetic groupings showed some
      correlation with geographic location, and clearly demonstrated a low level of
      host species-specificity within these viruses.
FAU - Ahne, W
AU  - Ahne W
AD  - Institute of Zoology, Fishery Biology and Fish Diseases, University of Munich,
      Germany.
FAU - Batts, W N
AU  - Batts WN
FAU - Kurath, G
AU  - Kurath G
FAU - Winton, J R
AU  - Winton JR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (HN Protein)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Bothrops/genetics
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - HN Protein/genetics
MH  - Microscopy, Electron
MH  - Paramyxoviridae/classification/*genetics/isolation & purification/ultrastructure
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - RNA, Viral/genetics
MH  - Reptiles/*virology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Analysis, RNA
EDAT- 1999/10/06 00:00
MHDA- 1999/10/06 00:01
CRDT- 1999/10/06 00:00
PHST- 1999/10/06 00:00 [pubmed]
PHST- 1999/10/06 00:01 [medline]
PHST- 1999/10/06 00:00 [entrez]
AID - S0168-1702(99)00059-3 [pii]
AID - 10.1016/s0168-1702(99)00059-3 [doi]
PST - ppublish
SO  - Virus Res. 1999 Sep;63(1-2):65-74. doi: 10.1016/s0168-1702(99)00059-3.