PMID- 10508590
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20190728
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 9
IP  - 17
DP  - 1999 Sep 9
TI  - Regulation of type IIalpha phosphatidylinositol phosphate kinase localisation by 
      the protein kinase CK2.
PG  - 983-6
AB  - Inositol lipid synthesis is regulated by several distinct families of enzymes
      [1]. Members of one of these families, the type II phosphatidylinositol phosphate
      kinases (PIP kinases), are 4-kinases and are thought to catalyse a minor route of
      synthesis of the multifunctional phosphatidylinositol 4,5-bisphosphate
      (PI(4,5)P(2)) from the inositide PI(5)P [2]. Here, we demonstrate the partial
      purification of a protein kinase that phosphorylates the type IIalpha PIP kinase 
      at a single site unique to that isoform - Ser304. This kinase was identified as
      protein kinase CK2 (formerly casein kinase 2). Mutation of Ser304 to aspartate to
      mimic its phosphorylation had no effect on PIP kinase activity, but promoted both
      redistribution of the green fluorescent protein (GFP)-tagged enzyme in HeLa cells
      from the cytosol to the plasma membrane, and membrane ruffling. This effect was
      mimicked by mutation of Ser304 to alanine, although not to threonine, suggesting 
      a mechanism involving the unmasking of a latent membrane localisation sequence in
      response to phosphorylation.
FAU - Hinchliffe, K A
AU  - Hinchliffe KA
AD  - Department of Pharmacology Tennis Court Road, Cambridge, CB2 1QJ, UK.
      kh224@cam.ac.uk
FAU - Ciruela, A
AU  - Ciruela A
FAU - Letcher, A J
AU  - Letcher AJ
FAU - Divecha, N
AU  - Divecha N
FAU - Irvine, R F
AU  - Irvine RF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Luminescent Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 17885-08-4 (Phosphoserine)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.67 (phosphatidylinositol phosphate 4-kinase)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
EIN - Curr Biol. 2003 Jan 21;13(2):178.
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Casein Kinase II
MH  - Cell Membrane/enzymology/ultrastructure
MH  - Cytosol/metabolism
MH  - Genes, Reporter
MH  - Green Fluorescent Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Luminescent Proteins/genetics/metabolism
MH  - Membrane Proteins/metabolism
MH  - Microscopy, Confocal
MH  - Microscopy, Fluorescence
MH  - Minor Histocompatibility Antigens
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/physiology
MH  - Phosphorylation
MH  - Phosphoserine/chemistry
MH  - Phosphotransferases (Alcohol Group Acceptor)/*metabolism
MH  - Point Mutation
MH  - Protein Isoforms/chemistry/isolation & purification/*physiology
MH  - Protein Processing, Post-Translational
MH  - Protein-Serine-Threonine Kinases/chemistry/isolation & purification/*physiology
MH  - Recombinant Fusion Proteins/metabolism
EDAT- 1999/10/06 00:00
MHDA- 1999/10/06 00:01
CRDT- 1999/10/06 00:00
PHST- 1999/10/06 00:00 [pubmed]
PHST- 1999/10/06 00:01 [medline]
PHST- 1999/10/06 00:00 [entrez]
AID - S0960-9822(99)80429-1 [pii]
AID - 10.1016/s0960-9822(99)80429-1 [doi]
PST - ppublish
SO  - Curr Biol. 1999 Sep 9;9(17):983-6. doi: 10.1016/s0960-9822(99)80429-1.