PMID- 10508527
OWN - NLM
STAT- MEDLINE
DCOM- 19991019
LR  - 20071115
IS  - 1061-4036 (Print)
IS  - 1061-4036 (Linking)
VI  - 23
IP  - 2
DP  - 1999 Oct
TI  - Loss of Cul1 results in early embryonic lethality and dysregulation of cyclin E.
PG  - 245-8
AB  - The sequential timing of cell-cycle transitions is primarily governed by the
      availability and activity of key cell-cycle proteins. Recent studies in yeast
      have identified a class of ubiquitin ligases (E3 enzymes) called SCF complexes,
      which regulate the abundance of proteins that promote and inhibit cell-cycle
      progression at the G1-S phase transition. SCF complexes consist of three
      invariable components, Skp1, Cul-1 (Cdc53 in yeast) and Rbx1, and a variable
      F-box protein that recruits a specific cellular protein to the ubquitin pathway
      for degradation. To study the role of Cul-1 in mammalian development and
      cell-cycle regulation, we generated mice deficient for Cul1 and analysed null
      embryos and heterozygous cell lines. We show that Cul1 is required for early
      mouse development and that Cul1 mutants fail to regulate the abundance of the G1 
      cyclin, cyclin E (encoded by Ccne), during embryogenesis.
FAU - Dealy, M J
AU  - Dealy MJ
AD  - Department of Biology, University of California, San Diego, California
      92093-0366, USA.
FAU - Nguyen, K V
AU  - Nguyen KV
FAU - Lo, J
AU  - Lo J
FAU - Gstaiger, M
AU  - Gstaiger M
FAU - Krek, W
AU  - Krek W
FAU - Elson, D
AU  - Elson D
FAU - Arbeit, J
AU  - Arbeit J
FAU - Kipreos, E T
AU  - Kipreos ET
FAU - Johnson, R S
AU  - Johnson RS
LA  - eng
SI  - GENBANK/AF136441
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Nat Genet
JT  - Nature genetics
JID - 9216904
RN  - 0 (Cdc53 protein, S cerevisiae)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cullin Proteins)
RN  - 0 (Cyclin E)
RN  - 0 (RNA, Messenger)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Animals
MH  - Cell Cycle Proteins/*genetics
MH  - Cell Death/genetics
MH  - Cell Division/genetics
MH  - Cells, Cultured
MH  - *Cullin Proteins
MH  - Cyclin E/*genetics/metabolism
MH  - Embryo, Mammalian/cytology/metabolism
MH  - Embryonic and Fetal Development
MH  - Female
MH  - Fetal Death/*genetics
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Pregnancy
MH  - RNA, Messenger/genetics/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Tumor Suppressor Protein p53/analysis
EDAT- 1999/10/03 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/10/03 09:00
PHST- 1999/10/03 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/10/03 09:00 [entrez]
AID - 10.1038/13886 [doi]
PST - ppublish
SO  - Nat Genet. 1999 Oct;23(2):245-8. doi: 10.1038/13886.