PMID- 10506931
OWN - NLM
STAT- MEDLINE
DCOM- 19991126
LR  - 20180607
IS  - 0213-3911 (Print)
IS  - 0213-3911 (Linking)
VI  - 14
IP  - 4
DP  - 1999 Oct
TI  - Immunoreactivity of Thomsen-Friedenreich (TF) antigen in human neoplasms: the
      importance of carrier-specific glycotope expression on MUC1.
PG  - 1153-8
LID - 10.14670/HH-14.1153 [doi]
AB  - On the basis of their known fine specificities we evaluated the
      immunohistochemical marker qualities of two monoclonal antibodies (mabs) defining
      the tumor-associated TF disaccharide Gal beta 1-3 GalNAc. This antigen is
      expressed in certain tumors in correlation with prognosis and metastasis. The
      reactivity of one of these mabs (A78-G/A7) depends on clustered TF disaccharides 
      (glycosylation at vicinal Ser/Thr positions) while the other--mab BW835--has been
      characterized to bind specifically to TF disaccharide linked to a motif within
      the MUC1 repeat. Therefore, mab BW835 represents an interesting tool for the
      identification of tumor-associated glycoforms of MUC1, which are involved in
      tumor progression and metastasis, but also in the recognition of tumor cells by
      cytotoxic T cells. As references the TF-binding lectins from peanut (PNA) and
      Artocarpus integrifolia (jacalin) were applied. The binding patterns of these
      immunoreagents were strikingly distinct. Mab BW835 showed a significantly
      stronger reactivity than mab A78-G/A7, especially in gastric, mammary,
      pancreatic, thyreoideal, renal and bladder carcinomas. PNA and jacalin receptors 
      exhibited an expression in the majority of all cancer types, with the exception
      of seminoma and glioblastoma/sarcoma. These results can be explained by the
      broader fine specificities of the lectins. Furthermore, a strong expression of
      MUC1-bound TF antigen is indicated by the staining pattern of mab BW835. The
      marker qualities of both antigens, TF and MUC1, are combined in the binding
      specificity of BW835, and hence this antibody may have a high impact for the
      immunodetection of these tumor-associated antigens.
FAU - Baldus, S E
AU  - Baldus SE
AD  - Institute of Pathology, University of Cologne, Germany. s-e.baldus@uni-koeln.de
FAU - Hanisch, F G
AU  - Hanisch FG
FAU - Monaca, E
AU  - Monaca E
FAU - Karsten, U R
AU  - Karsten UR
FAU - Zirbes, T K
AU  - Zirbes TK
FAU - Thiele, J
AU  - Thiele J
FAU - Dienes, H P
AU  - Dienes HP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - Histol Histopathol
JT  - Histology and histopathology
JID - 8609357
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Glycoproteins)
RN  - 0 (MUC1 tandem repeat peptide)
RN  - 0 (Mucin-1)
RN  - 0 (Peptide Fragments)
RN  - 0 (Thomsen-Friedenreich antigen, cryptic)
RN  - 0 (Trisaccharides)
SB  - IM
MH  - Epitopes, B-Lymphocyte/immunology
MH  - Glycoproteins/immunology
MH  - Humans
MH  - Mucin-1/*immunology
MH  - Neoplasms/*immunology/pathology
MH  - Peptide Fragments/*immunology
MH  - Trisaccharides/*immunology
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - 10.14670/HH-14.1153 [doi]
PST - ppublish
SO  - Histol Histopathol. 1999 Oct;14(4):1153-8. doi: 10.14670/HH-14.1153.