PMID- 10506216
OWN - NLM
STAT- MEDLINE
DCOM- 19991109
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 41
DP  - 1999 Oct 8
TI  - Characterization of the Shank family of synaptic proteins. Multiple genes,
      alternative splicing, and differential expression in brain and development.
PG  - 29510-8
AB  - Shank1, Shank2, and Shank3 constitute a family of proteins that may function as
      molecular scaffolds in the postsynaptic density (PSD). Shank directly interacts
      with GKAP and Homer, thus potentially bridging the N-methyl-D-aspartate
      receptor-PSD-95-GKAP complex and the mGluR-Homer complex in synapses (Naisbitt,
      S., Kim, E., Tu, J. C. , Xiao, B., Sala, S., Valtschanoff, J., Weinberg, R. J.,
      Worley, P. F., and Sheng, M. (1999) Neuron 23, 569-582; Tu, J. C., Xiao, B.,
      Naisbitt, S., Yuan, J. P., Petralia, R. S., Brakeman, P., Doan, A., Aakalu, V.
      K., Lanahan, A. A., Sheng, M., and Worley, P. F. (1999) Neuron 23, 583-592).
      Shank contains multiple domains for protein-protein interaction including ankyrin
      repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile alpha motif domain,
      and a proline-rich region. By characterizing Shank cDNA clones and RT-PCR
      products, we found that there are four sites for alternative splicing in Shank1
      and another four sites in Shank2, some of which result in deletion of specific
      domains of the Shank protein. In addition, the expression of the splice variants 
      is differentially regulated in different regions of rat brain during development.
      Immunoblot analysis of Shank proteins in rat brain using five different Shank
      antibodies reveals marked heterogeneity in size (120-240 kDa) and differential
      spatiotemporal expression. Shank1 immunoreactivity is concentrated at excitatory 
      synaptic sites in adult brain, and the punctate staining of Shank1 is seen in
      developing rat brains as early as postnatal day 7. These results suggest that
      alternative splicing in the Shank family may be a mechanism that regulates the
      molecular structure of Shank and the spectrum of Shank-interacting proteins in
      the PSDs of adult and developing brain.
FAU - Lim, S
AU  - Lim S
AD  - Department of Pharmacology, Pusan National University, Kumjeong-ku, Pusan
      609-735, Korea.
FAU - Naisbitt, S
AU  - Naisbitt S
FAU - Yoon, J
AU  - Yoon J
FAU - Hwang, J I
AU  - Hwang JI
FAU - Suh, P G
AU  - Suh PG
FAU - Sheng, M
AU  - Sheng M
FAU - Kim, E
AU  - Kim E
LA  - eng
SI  - GENBANK/AF141901
SI  - GENBANK/AF141902
SI  - GENBANK/AF141903
SI  - GENBANK/AF141904
GR  - NS35050/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SHANK3 protein, human)
RN  - 0 (Shank1 protein, rat)
RN  - 0 (Shank2 protein, rat)
RN  - 0 (Shank3 protein, rat)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/growth & development/*metabolism
MH  - COS Cells
MH  - Carrier Proteins/chemistry/*genetics
MH  - Cloning, Molecular
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation, Developmental/genetics
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/chemistry/*genetics/immunology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Synaptic Transmission/*genetics
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - 10.1074/jbc.274.41.29510 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 8;274(41):29510-8. doi: 10.1074/jbc.274.41.29510.