PMID- 10506182
OWN - NLM
STAT- MEDLINE
DCOM- 19991109
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 41
DP  - 1999 Oct 8
TI  - MDC-L, a novel metalloprotease disintegrin cysteine-rich protein family member
      expressed by human lymphocytes.
PG  - 29251-9
AB  - The metalloprotease disintegrin cysteine-rich (MDC) proteins are a recently
      identified family of transmembrane proteins that function in proteolytic
      processing of cell surface molecules and in cell adhesion. Since lymphocytes must
      interact with a constantly changing environment, we hypothesized that lymphocytes
      would express unique MDC proteins. To identify MDC proteins expressed in human
      lymph node, a polymerase chain reaction-based strategy combined with degenerate
      oligonucleotide primers was employed. We report here the identification of MDC-L 
      (ADAM 23), a novel member of the MDC protein family. The results obtained from
      cDNA cloning and Northern blot analysis of mRNA isolated from various lymphoid
      tissues indicate that a 2.8-kilobase mRNA encoding a transmembrane form, MDC-Lm, 
      and a 2.2-kilobase mRNA encoding a secreted form, MDC-Ls, are expressed in a
      tissue-specific manner. MDC-L mRNA was shown to be predominantly expressed in
      secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach,
      colon, appendix, and trachea. Furthermore, immunohistochemical staining with an
      anti-MDC-L antibody demonstrated that cells with typical lymphocyte morphology
      are responsible for expression of the MDC-L antigen in these lymphoid tissues.
      MDC-Lm was found to be expressed on the surface of human peripheral blood
      lymphocytes and transformed B- and T-lymphocyte cell lines as an 87-kDa protein. 
      Thus, we have identified a novel lymphocyte-expressed MDC protein family member.
FAU - Roberts, C M
AU  - Roberts CM
AD  - Department of Biochemistry, University of Oklahoma Health Sciences Center,
      Oklahoma City, Oklahoma 73190, USA.
FAU - Tani, P H
AU  - Tani PH
FAU - Bridges, L C
AU  - Bridges LC
FAU - Laszik, Z
AU  - Laszik Z
FAU - Bowditch, R D
AU  - Bowditch RD
LA  - eng
SI  - GENBANK/AF137334
SI  - GENBANK/AF137335
GR  - GM51616/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Disintegrins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (Adam23 protein, mouse)
RN  - EC 3.4.24.- (Metalloendopeptidases)
SB  - IM
MH  - ADAM Proteins
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - *Disintegrins
MH  - Humans
MH  - Immunohistochemistry
MH  - Lymph Nodes
MH  - Lymphocytes
MH  - Metalloendopeptidases/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/chemistry/*genetics
MH  - RNA, Messenger/metabolism
MH  - Sequence Alignment
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - 10.1074/jbc.274.41.29251 [doi]
AID - S0021-9258(19)51968-8 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 8;274(41):29251-9. doi: 10.1074/jbc.274.41.29251.