PMID- 10506177
OWN - NLM
STAT- MEDLINE
DCOM- 19991109
LR  - 20220309
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 41
DP  - 1999 Oct 8
TI  - The quaking I-5 protein (QKI-5) has a novel nuclear localization signal and
      shuttles between the nucleus and the cytoplasm.
PG  - 29202-10
AB  - The mouse quaking (qk) gene is essential in both myelination and early
      embryogenesis. Its product, QKI, is an RNA-binding protein belonging to a growing
      protein family called STAR (signal transduction and activator of RNA). All
      members have an approximately 200-amino acid STAR domain, which contains a single
      extended heteronuclear ribonucleoprotein K homologue domain flanked by two
      domains called QUA1 and QUA2. We found that QKI isoforms could associate with
      each other, while one of the lethal mutations qkI(kt4) with a single amino acid
      change in QUA1 domain, leads to a loss of QKI self-interaction. This suggests
      that the QUA1 domain is responsible for QKI dimerization. Three QKI isoforms have
      different carboxyl termini and different subcellular localization. Here, using
      GFP fusion protein, we identified a 7-amino acid novel nuclear localization
      sequence in the carboxyl terminus of QKI-5, which is conserved in a subclass of
      STAR proteins containing SAM68 and ETLE/T-STAR. Thus, we name this motif
      STAR-NLS. In addition, the effects of active transcription, RNA-binding and
      self-interaction on QKI-5 localization were analyzed. Furthermore, using an
      interspecies heterokaryon assay, we found that QKI-5, but not another STAR
      protein ETLE, shuttles between the nucleus and the cytoplasm, which suggests that
      QKI-5 functions in both cell compartments.
FAU - Wu, J
AU  - Wu J
AD  - Institute for Cellular and Molecular Biology, Department of Microbiology,
      University of Texas, Austin, Texas 78712-1064, USA.
FAU - Zhou, L
AU  - Zhou L
FAU - Tonissen, K
AU  - Tonissen K
FAU - Tee, R
AU  - Tee R
FAU - Artzt, K
AU  - Artzt K
LA  - eng
GR  - HD10668/HD/NICHD NIH HHS/United States
GR  - HD30658/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (QKI protein, human)
RN  - 0 (Qk protein, mouse)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Dimerization
MH  - Genes, Lethal
MH  - Green Fluorescent Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Immunohistochemistry
MH  - Luminescent Proteins
MH  - Mice
MH  - Mice, Quaking
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Nuclear Localization Signals/*genetics
MH  - RNA-Binding Proteins/*metabolism
MH  - Recombinant Fusion Proteins
MH  - Signal Transduction
MH  - Transfection
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - 10.1074/jbc.274.41.29202 [doi]
AID - S0021-9258(19)51963-9 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 8;274(41):29202-10. doi: 10.1074/jbc.274.41.29202.