PMID- 10506124
OWN - NLM
STAT- MEDLINE
DCOM- 19991109
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 41
DP  - 1999 Oct 8
TI  - Identification of an amino acid transporter associated with the
      cystinuria-related type II membrane glycoprotein.
PG  - 28845-8
AB  - We identified an amino acid transporter that is associated with the
      cystinuria-related type II membrane glycoprotein, rBAT (related to b(0,+) amino
      acid transporter). The transporter designated BAT1 (b(0, +)-type amino acid
      transporter 1) from rat kidney was found to be structurally related to recently
      identified amino acid transporters for system L, system y(+)L, and system x(-)C, 
      which are linked, via a disulfide bond, to the other type II membrane
      glycoprotein, 4F2hc (4F2 heavy chain). In the nonreducing condition, a 125-kDa
      band, which seems to correspond to the heterodimeric complex of BAT1 and rBAT,
      was detected in rat kidney with anti-BAT1 antibody. The band was shifted to 41
      kDa in the reducing condition, confirming that BAT1 and rBAT are linked via a
      disulfide bond. The BAT1 and rBAT proteins were shown to be colocalized in the
      apical membrane of the renal proximal tubules where massive cystine transport had
      been proposed. When expressed in COS-7 cells with rBAT, but not with 4F2hc, BAT1 
      exhibited a Na(+)-independent transport of cystine as well as basic and neutral
      amino acids with the properties of system b(0,+). The results from the present
      investigation were used to establish a family of amino acid transporters
      associated with type II membrane glycoproteins.
FAU - Chairoungdua, A
AU  - Chairoungdua A
AD  - Department of Pharmacology, Kyorin University School of Medicine, 6-20-2
      Shinkawa, Mitaka, Tokyo 181-8611, USA.
FAU - Segawa, H
AU  - Segawa H
FAU - Kim, J Y
AU  - Kim JY
FAU - Miyamoto, K
AU  - Miyamoto K
FAU - Haga, H
AU  - Haga H
FAU - Fukui, Y
AU  - Fukui Y
FAU - Mizoguchi, K
AU  - Mizoguchi K
FAU - Ito, H
AU  - Ito H
FAU - Takeda, E
AU  - Takeda E
FAU - Endou, H
AU  - Endou H
FAU - Kanai, Y
AU  - Kanai Y
LA  - eng
SI  - GENBANK/AB029559
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Amino Acid Transport Systems, Basic)
RN  - 0 (Amino Acids)
RN  - 0 (Antigens, CD)
RN  - 0 (Carrier Proteins)
RN  - 0 (Disulfides)
RN  - 0 (Fusion Regulatory Protein-1)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - ATP-Binding Cassette Transporters/chemistry/genetics
MH  - Amino Acid Sequence
MH  - *Amino Acid Transport Systems, Basic
MH  - Amino Acids/metabolism
MH  - Animals
MH  - Antigens, CD/genetics
MH  - Biological Transport
MH  - COS Cells
MH  - Carrier Proteins/chemistry/*genetics
MH  - Cloning, Molecular
MH  - Cystinuria/*genetics
MH  - Dimerization
MH  - Disulfides
MH  - Fusion Regulatory Protein-1
MH  - Immunohistochemistry
MH  - Kidney/metabolism
MH  - Membrane Glycoproteins/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Substrate Specificity
MH  - Transfection
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - 10.1074/jbc.274.41.28845 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Oct 8;274(41):28845-8. doi: 10.1074/jbc.274.41.28845.