PMID- 10506102
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190513
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 20
IP  - 10
DP  - 1999 Oct
TI  - Alterations of SAG mRNA in human cancer cell lines: requirement for the RING
      finger domain for apoptosis protection.
PG  - 1899-903
AB  - We have recently cloned and characterized a novel zinc RING finger gene, SAG
      (sensitive to apoptosis gene). SAG has antioxidant activity and protects cells
      from apoptosis induced by metal ions and redox compounds. During the course of
      SAG cloning, we identified two forms of deletions of SAG mRNA in colon and
      testicular carcinoma cell lines. The first form (SAG-MU1) consists of a 7 bp
      deletion that results in a frameshift and abolishes the RING finger domain. The
      second (SAG-MU2) consists of an in-frame 48 bp deletion that truncates 16 amino
      acids in the protein but retains the RING finger domain. Functional studies using
      stable transfectant lines reveal that, like wild-type SAG, SAG-MU2 retains
      anti-apoptosis activity, whereas SAG-MU1 shows no such protection. The results
      indicate that the C-terminal zinc RING finger domain is required for
      anti-apoptosis activity of SAG.
FAU - Sun, Y
AU  - Sun Y
AD  - Department of Molecular Biology, Parke-Davis Pharmaceutical Research, Division of
      Warner-Lambert Co., Ann Arbor, MI 48105, USA. yi.sun@wl.com
LA  - eng
PT  - Journal Article
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (DNA Primers)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 2.3.2.27 (RNF7 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Apoptosis/*genetics
MH  - Base Sequence
MH  - Colonic Neoplasms/*genetics/pathology
MH  - DNA Primers
MH  - Free Radical Scavengers/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - RNA, Messenger/*genetics
MH  - *RNA-Binding Proteins
MH  - Sequence Deletion
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
MH  - Ubiquitin-Protein Ligases
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - 10.1093/carcin/20.10.1899 [doi]
PST - ppublish
SO  - Carcinogenesis. 1999 Oct;20(10):1899-903. doi: 10.1093/carcin/20.10.1899.