PMID- 10505793
OWN - NLM
STAT- MEDLINE
DCOM- 19991020
LR  - 20061115
IS  - 0829-8211 (Print)
IS  - 0829-8211 (Linking)
VI  - 77
IP  - 3
DP  - 1999
TI  - Molecular cloning and expression of rat antisecretory factor and its
      intracellular localization.
PG  - 223-8
AB  - Antisecretory factor (AF) was identified as a pituitary protein that inhibits the
      intestinal fluid secretion induced by cholera toxin. One aim of this study was to
      elucidate whether AF is also synthesized in the intestine or if AF produced in
      the pituitary is transported to the intestinal tract for its function there. cDNA
      clones encoding a protein proposed to be AF were isolated from rat pituitary
      gland and intestinal mucosa cDNA libraries. The nucleotide sequences of clones
      isolated from the rat pituitary gland and intestinal mucosa were identical. The
      deduced amino acid sequence was highly homologous to the sequence for subunit 5a 
      of the human 26S protease that exists abundantly in the cytosol and nucleus. The 
      production of AF in the intestine was confirmed by Northern blot and immunoblot
      analyses. Immunocytochemical observations of cells transfected with the rat AF
      cDNA showed that the AF protein was localized in the cytoplasm. These findings
      suggest that the protein proposed to be AF may be a cytoplasmic protein, it
      exists in the intestine rather than being transported from the pituitary gland,
      and it may function in intestinal cells.
FAU - Tateishi, K
AU  - Tateishi K
AD  - Department of Biochemistry, Fukuoka University School of Medicine, Japan.
      tateishi@msat.fukuoka-u.ac.jp
FAU - Misumi, Y
AU  - Misumi Y
FAU - Ikehara, Y
AU  - Ikehara Y
FAU - Miyasaka, K
AU  - Miyasaka K
FAU - Funakoshi, A
AU  - Funakoshi A
LA  - eng
SI  - GENBANK/AB017188
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (DNA, Complementary)
RN  - 0 (Neuropeptides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (antisecretory factor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - Intestinal Mucosa/*metabolism
MH  - Molecular Sequence Data
MH  - Neuropeptides/*genetics/metabolism
MH  - Pituitary Gland/*metabolism
MH  - Rats
MH  - Recombinant Proteins/genetics/metabolism
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
PST - ppublish
SO  - Biochem Cell Biol. 1999;77(3):223-8.