PMID- 10505033
OWN - NLM
STAT- MEDLINE
DCOM- 19991007
LR  - 20190718
IS  - 0959-8049 (Print)
IS  - 0959-8049 (Linking)
VI  - 35
IP  - 5
DP  - 1999 May
TI  - Significant increase of a specific variant TSG101 transcript during the
      progression of cervical neoplasia.
PG  - 733-7
AB  - The human tumour susceptibility gene TSG101 has recently been identified on
      chromosomal locus 11p15.1-15.2 which is frequently affected by genetic
      alterations in neoplastic lesions of the uterine cervix. Aberrant transcripts of 
      the TSG101 gene have been reported in various tumour entities, including breast, 
      ovarian and prostate cancers, but also in several non-neoplastic tissues. We
      analysed TSG101 transcription by reverse transcription-polymerase chain reaction 
      (RT-PCR) in a total of 139 clinical samples of cervical tissues and in cervical
      carcinoma cell lines. Variant transcripts were observed in all cell lines, in 69 
      of 122 (57%) cervical dysplasia and carcinoma samples and in five of 17 (29%)
      normal cervical tissues. One specific variant TSG101 transcript (delta 154-1054) 
      was detected with a significantly increased frequency in advanced preneoplastic
      cervical lesions. However, the relative abundance of variant TSG101 transcripts
      appeared to be generally low, as only wild-type, but no variant transcripts were 
      detectable in Northern blot analyses of cervical carcinoma cell lines. These data
      point to a progressive loss of stringent splice control functions or to extended 
      alternative splicing in advanced dysplasia and neoplasia. The relative amounts of
      variant transcripts do not support a major functional role of TSG101 variants in 
      cervical carcinogenesis.
FAU - Klaes, R
AU  - Klaes R
AD  - Department of Surgery, University of Heidelberg, Germany.
FAU - Kloor, M
AU  - Kloor M
FAU - Willeke, F
AU  - Willeke F
FAU - Melsheimer, P
AU  - Melsheimer P
FAU - von Knebel Doeberitz, M
AU  - von Knebel Doeberitz M
FAU - Ridder, R
AU  - Ridder R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Cancer
JT  - European journal of cancer (Oxford, England : 1990)
JID - 9005373
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Tsg101 protein)
SB  - IM
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Cervical Intraepithelial Neoplasia/*genetics
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - Disease Progression
MH  - Endosomal Sorting Complexes Required for Transport
MH  - Female
MH  - Humans
MH  - Neoplasm Proteins/*genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription Factors/*genetics/metabolism
MH  - Transcription, Genetic
MH  - Uterine Cervical Dysplasia/*genetics
MH  - Uterine Cervical Neoplasms/*genetics
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - S0959804999000167 [pii]
AID - 10.1016/s0959-8049(99)00016-7 [doi]
PST - ppublish
SO  - Eur J Cancer. 1999 May;35(5):733-7. doi: 10.1016/s0959-8049(99)00016-7.