PMID- 10504389
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 265
IP  - 2
DP  - 1999 Oct
TI  - Molecular cloning and expression of Galbeta1,3GalNAc alpha2, 3-sialyltransferase 
      from human fetal liver.
PG  - 580-8
AB  - Based on the sequences of the highly conserved segments in the previously cloned 
      sialyltransferases, a cDNA encoding Galbeta1, 3GalNAc alpha2,3-sialyltransferase 
      (SIATFL) has been isolated from human fetal liver. Expression analysis of the
      gene has been performed with various carcinoma cell lines, fetal tissues, fetal
      and adult liver and both hepatoma and the surrounding tissue from the same liver.
      The SIATFL gene was expressed poorly in fetal liver and in adult liver, slightly 
      in hepatoma and highly in the surrounding tissue of hepatoma. The cDNA encoding
      the putative active domain was expressed in COS-1, Escherichia coli, and Pichia
      pastoris. The recombinant protein expressed in COS-1 could catalyse the transfer 
      of NeuAc from CMP-NeuAc to asialo-fetuin. No enzyme activity was detected with a 
      32-kDa protein in E. coli and both 32-kDa and 41-kDa proteins in P. pastoris.
      These results suggested that correct glycosylation of the enzyme might play a key
      role in its folding that may be directly related to the enzymatic activity.
FAU - Shang, J
AU  - Shang J
AD  - State Key Laboratory of Microbial Resources, Institute of Microbiology,Academia
      Sinica, Beijing, China.
FAU - Qiu, R
AU  - Qiu R
FAU - Wang, J
AU  - Wang J
FAU - Liu, J
AU  - Liu J
FAU - Zhou, R
AU  - Zhou R
FAU - Ding, H
AU  - Ding H
FAU - Yang, S
AU  - Yang S
FAU - Zhang, S
AU  - Zhang S
FAU - Jin, C
AU  - Jin C
LA  - eng
SI  - GENBANK/AF059321
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Asialoglycoproteins)
RN  - 0 (Fetuins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (alpha-Fetoproteins)
RN  - 0 (asialofetuin)
RN  - 3063-71-6 (Cytidine Monophosphate N-Acetylneuraminic Acid)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - EC 2.4.99.4 (beta-galactoside alpha-2,3-sialyltransferase)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Asialoglycoproteins/metabolism
MH  - Base Sequence
MH  - Binding Sites
MH  - COS Cells
MH  - Cloning, Molecular
MH  - Cytidine Monophosphate N-Acetylneuraminic Acid/metabolism
MH  - Escherichia coli
MH  - Fetuins
MH  - *Gene Expression Regulation, Enzymologic
MH  - Glycosylation
MH  - Humans
MH  - Liver/embryology/*enzymology
MH  - Molecular Sequence Data
MH  - N-Acetylneuraminic Acid/metabolism
MH  - Pichia
MH  - Protein Folding
MH  - Recombinant Proteins/chemistry/genetics
MH  - Sequence Homology
MH  - Sialyltransferases/chemistry/*genetics
MH  - alpha-Fetoproteins/metabolism
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - ejb733 [pii]
AID - 10.1046/j.1432-1327.1999.00733.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Oct;265(2):580-8. doi: 10.1046/j.1432-1327.1999.00733.x.