PMID- 10504338
OWN - NLM
STAT- MEDLINE
DCOM- 19991217
LR  - 20191210
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 19)
DP  - 1999 Oct
TI  - Interaction between the Ret finger protein and the Int-6 gene product and
      co-localisation into nuclear bodies.
PG  - 3331-42
AB  - The mouse int-6 gene was identified in mammary tumors as an integration site for 
      the mouse mammary tumor virus. Its human counterpart encodes a product that
      interacts with the Tax viral oncoprotein of the human T cell leukaemia virus type
      1. This interaction impedes the localisation of over-expressed Int-6 in nuclear
      bodies containing the promyelocytic leukaemia gene product (PML). In this study, 
      Int-6 is characterised as a 52 kDa protein that is localised within nuclear
      bodies in primary lymphocytes. Screening of a human B cell cDNA library for
      proteins that interact with Int-6 led to isolation of four clones coding for the 
      p110 subunit of eIF3, in accordance with previous detection of Int-6 in purified 
      forms of this translation initiation factor. Another clone was interesting with
      respect to the subcellular localisation of Int-6. It encodes the Ret finger
      protein (Rfp) which interacts with PML and localises within a subset of PML
      nuclear bodies. The interaction of Rfp with Int-6 is mediated through a region in
      Rfp designated 'Rfp domain', distinct from that involved in the interaction with 
      PML. Int-6 and Rfp are co-localised in certain PML nuclear bodies in lymphocytes 
      and transfection studies in HeLa cells strongly suggest that Rfp triggers
      translocation of Int-6 to nuclear bodies.
FAU - Morris-Desbois, C
AU  - Morris-Desbois C
AD  - Laboratoire de Biologie Moleculaire et Cellulaire, UMR 5665 CNRS-ENSL, Allee
      d'Italie, France. pjalinot@ens-lyon.fr
FAU - Bochard, V
AU  - Bochard V
FAU - Reynaud, C
AU  - Reynaud C
FAU - Jalinot, P
AU  - Jalinot P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Eukaryotic Initiation Factor-3)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (TRIM27 protein, human)
RN  - EC 2.3.2.27 (Trim27 protein, mouse)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - COS Cells
MH  - Cell Nucleus/*chemistry/genetics
MH  - *DNA-Binding Proteins
MH  - Eukaryotic Initiation Factor-3
MH  - Fluorescent Antibody Technique
MH  - Gene Deletion
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis/physiology
MH  - Nuclear Proteins/chemistry/*genetics/*metabolism
MH  - Protein Structure, Tertiary
MH  - Proto-Oncogene Proteins/chemistry/*genetics/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Transfection
MH  - Two-Hybrid System Techniques
MH  - Ubiquitin-Protein Ligases
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
PST - ppublish
SO  - J Cell Sci. 1999 Oct;112 ( Pt 19):3331-42.