PMID- 10504327
OWN - NLM
STAT- MEDLINE
DCOM- 19991217
LR  - 20131121
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 19)
DP  - 1999 Oct
TI  - Overexpression of kin17 protein disrupts nuclear morphology and inhibits the
      growth of mammalian cells.
PG  - 3215-24
AB  - UVC or ionizing radiation of mammalian cells elicits a complex genetic response
      that allows recovery and cell survival. Kin17 gene, which is highly conserved
      among mammals, is upregulated during this response. Kin17 gene encodes a 45 kDa
      protein which binds to DNA and presents a limited similarity with a functional
      domain of the bacterial RecA protein. Kin17 protein is accumulated in the nucleus
      of proliferating fibroblasts and forms intranuclear foci. Using expression
      vectors, we show that overexpression of kin17 protein inhibits cell-cycle
      progression into S phase. Our results indicate that growth inhibition correlates 
      with disruption of the nuclear morphology which seems to modify the intranuclear 
      network required during the early steps of DNA replication. We report that a
      mutant encoding a protein deleted from the central domain of kin17 protein
      enhanced these effects whereas the deletion of the C-terminal domain considerably
      reduced them. These mutants will be used to elucidate the molecular mechanism by 
      which kin17 protein alters cell growth and DNA replication.
FAU - Kannouche, P
AU  - Kannouche P
AD  - Laboratoire de Genetique de la Radiosensibilite, Departement de Radiobiologie et 
      de Radiopathologie, Direction des Sciences du Vivant, Centre d'Etudes de
      Fontenay-aux-Roses, CEA, avenue du General-Leclerc, B.P. no. 6, France.
FAU - Angulo, J F
AU  - Angulo JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Antigens, Polyomavirus Transforming)
RN  - 0 (Chromatin)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KIN protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 2.7.7.- (Rec A Recombinases)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Antigens, Polyomavirus Transforming/genetics
MH  - Bromodeoxyuridine/analysis
MH  - Carcinoma, Non-Small-Cell Lung
MH  - Cell Division/genetics
MH  - Cell Nucleus/chemistry/*genetics
MH  - Chromatin/genetics
MH  - DNA Replication/*physiology
MH  - DNA, Complementary/genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Extrachromosomal Inheritance
MH  - Gene Deletion
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genes, Dominant
MH  - HeLa Cells
MH  - Humans
MH  - Lung Neoplasms
MH  - Mammals
MH  - Mutagenesis/physiology
MH  - *Nuclear Proteins
MH  - Nucleic Acid Conformation
MH  - RNA-Binding Proteins
MH  - Rec A Recombinases/genetics
MH  - S Phase/genetics
MH  - Zinc Fingers/genetics
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
PST - ppublish
SO  - J Cell Sci. 1999 Oct;112 ( Pt 19):3215-24.