PMID- 10504302
OWN - NLM
STAT- MEDLINE
DCOM- 20000110
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 20)
DP  - 1999 Oct
TI  - A fraction of mouse sperm chromatin is organized in nucleosomal hypersensitive
      domains enriched in retroposon DNA.
PG  - 3537-48
AB  - We have characterized a nuclease hypersensitive chromatin fraction from murine
      spermatozoa. Endogenous nuclease activity can be induced in mouse epididymal
      spermatozoa by appropriate stimuli and cause the localized degradation of
      chromosomal DNA. Based on these observations, we have isolated nuclease
      hypersensitive chromatin regions released from spermatozoa in the supernatant of 
      pelleted sperm cells, and have cloned and characterized the DNA. Gel
      electrophoresis of end-labelled released DNA fragments showed a typical
      nucleosomal distribution. Peripherally distributed nucleohistones were visualized
      by immunofluorescence in sperm nuclei, and histones were identified by western
      blot in sperm chromatin. Moreover, the released DNA is enriched in retroposon DNA
      from a variety of families. FISH and immunofluorescence analysis showed that
      retroposon DNA and nucleohistone chromatin co-localize and are both
      peripherically distributed in nuclei of spermatozoa. In contrast, a major
      satellite DNA probe, used for control, co-localizes with highly condensed
      chromatin in the central region of sperm nuclei. The nuclear Ran and RCC1
      proteins were also visualized in the dorsal margin of sperm nuclei, and were
      abundantly released with the hypersensitive chromatin fraction. Together, these
      results indicate that nucleohistone chromatin fraction(s) with typical features
      of 'active' chromatin are present in murine spermatozoa, are hypersensitive to
      nuclease cleavage, enriched in retroposon DNA and organized in nucleosomal
      domains. These observations suggest that nucleohistone domains identify a
      fraction of the sperm genome which may be functional during early embryogenesis.
FAU - Pittoggi, C
AU  - Pittoggi C
AD  - Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Rome,
      Italy. cspadaf@tin.it
FAU - Renzi, L
AU  - Renzi L
FAU - Zaccagnini, G
AU  - Zaccagnini G
FAU - Cimini, D
AU  - Cimini D
FAU - Degrassi, F
AU  - Degrassi F
FAU - Giordano, R
AU  - Giordano R
FAU - Magnano, A R
AU  - Magnano AR
FAU - Lorenzini, R
AU  - Lorenzini R
FAU - Lavia, P
AU  - Lavia P
FAU - Spadafora, C
AU  - Spadafora C
LA  - eng
SI  - GENBANK/AJ006721
SI  - GENBANK/AJ006723
SI  - GENBANK/AJ006827
SI  - GENBANK/AJ006828
SI  - GENBANK/AJ006829
SI  - GENBANK/AJ133873
SI  - GENBANK/AJ133874
SI  - GENBANK/AJ133875
SI  - GENBANK/AJ133876
SI  - GENBANK/AJ133877
SI  - GENBANK/AJ133878
SI  - GENBANK/AJ133879
SI  - GENBANK/AJ133880
SI  - GENBANK/AJ133881
SI  - GENBANK/AJ133882
SI  - GENBANK/AJ133883
SI  - GENBANK/AJ133884
SI  - GENBANK/AJ238772
SI  - GENBANK/AJ238773
SI  - GENBANK/AJ238774
SI  - GENBANK/AJ238775
SI  - GENBANK/AJ238776
SI  - GENBANK/AJ238777
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Chromatin)
RN  - 0 (Nucleosomes)
RN  - 0 (Retroelements)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/physiology/ultrastructure
MH  - Chromatin/*ultrastructure
MH  - Epididymis/physiology
MH  - Genomic Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Nucleosomes/genetics/*ultrastructure
MH  - *Retroelements
MH  - Sperm Motility
MH  - Spermatozoa/physiology/*ultrastructure
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
AID - 10.1242/jcs.112.20.3537 [doi]
PST - ppublish
SO  - J Cell Sci. 1999 Oct;112 ( Pt 20):3537-48. doi: 10.1242/jcs.112.20.3537.