PMID- 10504293
OWN - NLM
STAT- MEDLINE
DCOM- 20000110
LR  - 20191008
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 112 ( Pt 20)
DP  - 1999 Oct
TI  - A dynamic connection between centromeres and ND10 proteins.
PG  - 3443-54
AB  - ND10, otherwise known as nuclear dots, PML nuclear bodies or PODs, are punctate
      foci in interphase nuclei that contain several cellular proteins. The functions
      of ND10 have not been well defined, but they are sensitive to external stimuli
      such as stress and virus infection, and they are disrupted in malignant
      promyelocytic leukaemia cells. Herpes simplex virus type 1 regulatory protein
      Vmw110 induces the proteasome-dependent degradation of ND10 component proteins
      PML and Sp100, particularly the species of these proteins which are covalently
      conjugated to the ubiquitin-like protein SUMO-1. We have recently reported that
      Vmw110 also induces the degradation of centromere protein CENP-C with consequent 
      disruption of centromere structure. These observations led us to examine whether 
      there were hitherto undetected connections between ND10 and centromeres. In this 
      paper we report that hDaxx and HP1 (which have been shown to interact with CENP-C
      and Sp100, respectively) are present in a proportion of both ND10 and interphase 
      centromeres. Furthermore, the proteasome inhibitor MG132 induced an association
      between centromeres and ND10 proteins PML and Sp100 in a significant number of
      cells in the G(2) phase of the cell cycle. These results imply that there is a
      dynamic, cell cycle regulated connection between centromeres and ND10 proteins
      which can be stabilised by inhibition of proteasome-mediated proteolysis.
FAU - Everett, R D
AU  - Everett RD
AD  - MRC Virology Unit, Church Street, Glasgow G11 5JR, Scotland UK.
      r.everett@vir.gla.ac.uk
FAU - Earnshaw, W C
AU  - Earnshaw WC
FAU - Pluta, A F
AU  - Pluta AF
FAU - Sternsdorf, T
AU  - Sternsdorf T
FAU - Ainsztein, A M
AU  - Ainsztein AM
FAU - Carmena, M
AU  - Carmena M
FAU - Ruchaud, S
AU  - Ruchaud S
FAU - Hsu, W L
AU  - Hsu WL
FAU - Orr, A
AU  - Orr A
LA  - eng
GR  - 073915/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Autoantigens)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (DAXX protein, human)
RN  - 0 (Interferon-alpha)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Leupeptins)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SUMO-1 Protein)
RN  - 0 (Ubiquitins)
RN  - 0 (centromere protein C)
RN  - 107283-02-3 (heterochromatin-specific nonhistone chromosomal protein HP-1)
RN  - 135844-47-2 (Sp100 protein, human)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - RF1P63GW3K (benzyloxycarbonylleucyl-leucyl-leucine aldehyde)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - *Antigens, Nuclear
MH  - Autoantigens/analysis/metabolism
MH  - Carrier Proteins/analysis/metabolism
MH  - Cell Cycle/physiology
MH  - Cell Cycle Proteins/analysis/*metabolism
MH  - Centromere/drug effects/*physiology/ultrastructure
MH  - Chromosomal Proteins, Non-Histone/analysis/metabolism
MH  - Cysteine Endopeptidases/metabolism
MH  - Cysteine Proteinase Inhibitors/pharmacology
MH  - Hot Temperature
MH  - Humans
MH  - Interferon-alpha/pharmacology
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Leupeptins/pharmacology
MH  - Multienzyme Complexes/metabolism
MH  - Nuclear Proteins/analysis/metabolism
MH  - Proteasome Endopeptidase Complex
MH  - Recombinant Proteins/analysis/metabolism
MH  - SUMO-1 Protein
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Ubiquitins/analysis
EDAT- 1999/10/03 00:00
MHDA- 1999/10/03 00:01
CRDT- 1999/10/03 00:00
PHST- 1999/10/03 00:00 [pubmed]
PHST- 1999/10/03 00:01 [medline]
PHST- 1999/10/03 00:00 [entrez]
PST - ppublish
SO  - J Cell Sci. 1999 Oct;112 ( Pt 20):3443-54.