PMID- 10502508 OWN - NLM STAT- MEDLINE DCOM- 19991021 LR - 20191210 IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 262 IP - 2 DP - 1999 Sep 30 TI - Sequence analysis of the Xestia c-nigrum granulovirus genome. PG - 277-97 AB - The nucleotide sequence of the Xestia c-nigrum granulovirus (XcGV) genome was determined and found to comprise 178,733 bases with a G+C content of 40.7%. It contained 181 putative genes of 150 nucleotides or greater that showed minimal overlap. Eighty-four of these putative genes, which collectively accounted for 43% of the genome, are homologs of genes previously identified in the Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) genome. These homologs showed on average 33% amino acid sequence identity to those from AcMNPV. Several genes reported to have major roles in AcMNPV biology including ie-2, gp64, and egt were not found in the XcGV genome. However, open reading frames with homology to DNA ligase, two DNA helicases (one similar to a yeast mitochondrial helicase and the other to a putative AcMNPV helicase), and four enhancins (virus enhancing factors) were found. In addition, several ORFs are repeated; there are 7 genes related to AcMNPV orf2, 4 genes related to AcMNPV orf145/150, and a number of repeated genes unique to XcGV. Eight major repeated sequences (XcGV hrs) that are similar to sequences found in the Trichoplusia ni GV genome (TnGV) were found. CI - Copyright 1999 Academic Press. FAU - Hayakawa, T AU - Hayakawa T AD - Department of Entomology, University of California, Davis, One Shields Avenue, Davis, California, 95616, USA. FAU - Ko, R AU - Ko R FAU - Okano, K AU - Okano K FAU - Seong, S I AU - Seong SI FAU - Goto, C AU - Goto C FAU - Maeda, S AU - Maeda S LA - eng SI - GENBANK/AF162221 PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (Viral Proteins) SB - IM MH - Amino Acid Sequence MH - Baculoviridae/chemistry/enzymology/*genetics MH - Base Sequence MH - Genes, Duplicate/genetics MH - Genes, Viral/*genetics MH - *Genome, Viral MH - Molecular Sequence Data MH - Nucleopolyhedroviruses/chemistry/enzymology/genetics MH - Open Reading Frames/genetics MH - Promoter Regions, Genetic/genetics MH - Repetitive Sequences, Nucleic Acid/genetics MH - Sequence Alignment MH - Sequence Analysis, DNA MH - Sequence Homology, Amino Acid MH - Viral Proteins/chemistry/genetics EDAT- 1999/09/30 00:00 MHDA- 1999/09/30 00:01 CRDT- 1999/09/30 00:00 PHST- 1999/09/30 00:00 [pubmed] PHST- 1999/09/30 00:01 [medline] PHST- 1999/09/30 00:00 [entrez] AID - 10.1006/viro.1999.9894 [doi] AID - S0042-6822(99)99894-X [pii] PST - ppublish SO - Virology. 1999 Sep 30;262(2):277-97. doi: 10.1006/viro.1999.9894.