PMID- 10501970
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20190905
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 10
IP  - 10
DP  - 1999 Oct
TI  - Conservation of the Caenorhabditis elegans timing gene clk-1 from yeast to human:
      a gene required for ubiquinone biosynthesis with potential implications for
      aging.
PG  - 1000-4
AB  - Mutations in the Caenorhabditis elegans gene clk-1 have a major effect on slowing
      development and increasing life span. The Saccharomyces cerevisiae homolog COQ7
      encodes a mitochondrial protein involved in ubiquinone biosynthesis and, hence,
      is required for respiration and gluconeogenesis. In this study, RT-PCR and 5'
      RACE were used to isolate both human and mouse clk-1/COQ7 homologs. Human CLK-1
      was mapped to Chr 16(p12-13.1) by Radiation Hybrid (RH) and fluorescence in situ 
      hybridization (FISH) methods. The number and location of human CLK1 introns were 
      determined, and the location of introns II and IV are the same as in C. elegans. 
      Northern blot analysis showed that three different isoforms of CLK-1 mRNA are
      present in several tissues and that the isoforms differ in the amount of
      expression. The functional equivalence of human CLK-1 to the yeast COQ7 homolog
      was tested by introducing either a single or multicopy plasmid containing human
      CLK-1 cDNA into yeast coq7 deletion strains and assaying for growth on a
      nonfermentable carbon source. The human CLK-1 gene was able to functionally
      complement yeast coq7 deletion mutants. The protein similarities and the
      conservation of function of the CLK-1/clk-1/COQ7 gene products suggest a
      potential link between the production of ubiquinone and aging.
FAU - Vajo, Z
AU  - Vajo Z
AD  - Medical Genetics Branch, National Human Genome Research Institute, National
      Institutes of Health, 10 Center Dr. MSC 1852, Bldg. 10, Room 10C101, Bethesda,
      Maryland 20892-1852, USA.
FAU - King, L M
AU  - King LM
FAU - Jonassen, T
AU  - Jonassen T
FAU - Wilkin, D J
AU  - Wilkin DJ
FAU - Ho, N
AU  - Ho N
FAU - Munnich, A
AU  - Munnich A
FAU - Clarke, C F
AU  - Clarke CF
FAU - Francomano, C A
AU  - Francomano CA
LA  - eng
SI  - GENBANK/AF053770
GR  - GM0785/GM/NIGMS NIH HHS/United States
GR  - GM45952/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (CLK-1 protein, C elegans)
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Helminth Proteins)
RN  - 0 (RNA, Messenger)
RN  - 1339-63-5 (Ubiquinone)
RN  - RRK47DEG6Q (ubiquinone 7)
SB  - IM
MH  - Aging/*genetics
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biological Clocks/genetics
MH  - Caenorhabditis elegans/*genetics
MH  - *Caenorhabditis elegans Proteins
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 16
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - Evolution, Molecular
MH  - Exons/genetics
MH  - Genetic Complementation Test
MH  - Helminth Proteins/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Introns/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - RNA, Messenger/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Ubiquinone/*biosynthesis/genetics
MH  - Yeasts
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - MG99-657 [pii]
AID - 10.1007/s003359901147 [doi]
PST - ppublish
SO  - Mamm Genome. 1999 Oct;10(10):1000-4. doi: 10.1007/s003359901147.