PMID- 10501967
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20191210
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 10
IP  - 10
DP  - 1999 Oct
TI  - Cloning of Fatso (Fto), a novel gene deleted by the Fused toes (Ft) mouse
      mutation.
PG  - 983-6
AB  - The Fused toes (Ft) mouse mutation was created by insertional mutagenesis,
      resulting in the deletion of several hundred kb of genomic sequences of mouse
      Chromosome (Chr) 8. Mice heterozygous for the Ft mutation are characterized by
      partial syndactyly of forelimbs and massive thymic hyperplasia indicating that
      programmed cell death is affected. Homozygous Ft/Ft embryos die at midgestation
      and show severe malformations of craniofacial structures. Furthermore,
      establishment of left-right asymmetry is random. Here we report on the positional
      cloning of a novel gene by exon trap analysis of a genomic clone encoding
      wild-type sequences corresponding to parts of the deletion in Ft mutants. RT-PCR 
      experiments demonstrated that the newly identified gene, Fatso (Fto), is
      expressed throughout embryonic development. Wide expression was also found in
      tissues of adult mice. We show that expression of Fto is completely absent in
      mouse embryonic fibroblasts homozygous for the Ft mutation. In addition, we
      isolated the full-length cDNA which encodes a putative 58-kDa protein showing no 
      similarities to known proteins or protein motifs. The expression data of Fto
      define it as a candidate gene involved in processes such as programmed cell
      death, craniofacial development, and establishment of left-right asymmetry.
FAU - Peters, T
AU  - Peters T
AD  - Institut fur Molekularbiologie, Medizinische Hochschule Hannover, 30625 Hannover,
      Germany.
FAU - Ausmeier, K
AU  - Ausmeier K
FAU - Ruther, U
AU  - Ruther U
LA  - eng
SI  - GENBANK/AJ237917
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Fts protein, mouse)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis/genetics
MH  - Apoptosis Regulatory Proteins
MH  - Base Sequence
MH  - Chromosomes/genetics
MH  - Cloning, Molecular
MH  - *Gene Deletion
MH  - Gene Expression Regulation, Developmental
MH  - Heterozygote
MH  - Homozygote
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Biosynthesis
MH  - Proteins/chemistry/*genetics
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transgenes/genetics
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - MG99-678 [pii]
AID - 10.1007/s003359901144 [doi]
PST - ppublish
SO  - Mamm Genome. 1999 Oct;10(10):983-6. doi: 10.1007/s003359901144.