PMID- 10501226
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20210218
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 73
IP  - 4
DP  - 1999 Oct
TI  - Phosphorylation of serine-880 in GluR2 by protein kinase C prevents its C
      terminus from binding with glutamate receptor-interacting protein.
PG  - 1765-8
AB  - Phosphorylation of the glutamate receptor is an important mechanism of synaptic
      plasticity. Here, we show that the C terminus of GluR2 of the
      alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptor is
      phosphorylated by protein kinase C and that serine-880 is the major
      phosphorylation site. This phosphorylation also occurs in human embryonic kidney 
      (HEK) cells by addition of 12-O-tetradecanoylphorbol 13-acetate. Our
      immunoprecipitation experiment revealed that the phosphorylation of serine-880 in
      GluR2 drastically reduced the affinity for glutamate receptor-interacting protein
      (GRIP), a synaptic PDZ domain-containing protein, in vitro and in HEK cells. This
      result suggests that modulation of serine-880 phosphorylation in GluR2 controls
      the clustering of AMPA receptors at excitatory synapses and consequently
      contributes to synaptic plasticity.
FAU - Matsuda, S
AU  - Matsuda S
AD  - Laboratory for Memory and Learning, RIKEN Brain Science Institute, Saitama,
      Japan.
FAU - Mikawa, S
AU  - Mikawa S
FAU - Hirai, H
AU  - Hirai H
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Antibodies)
RN  - 0 (Carrier Proteins)
RN  - 0 (GRIP1 protein, human)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, Metabotropic Glutamate)
RN  - 0 (Recombinant Proteins)
RN  - 0 (metabotropic glutamate receptor 2)
RN  - 17885-08-4 (Phosphoserine)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies
MH  - Binding Sites
MH  - Carrier Proteins/chemistry/*metabolism
MH  - Cell Line
MH  - Humans
MH  - Immunoblotting
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/chemistry/*metabolism
MH  - Peptide Fragments/chemistry/immunology
MH  - Phosphorylation
MH  - Phosphoserine/metabolism
MH  - Protein Kinase C/*metabolism
MH  - Receptors, AMPA/metabolism
MH  - Receptors, Metabotropic Glutamate/chemistry/genetics/*metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - *Serine
MH  - Synapses/physiology
MH  - Transfection
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - 10.1046/j.1471-4159.1999.731765.x [doi]
PST - ppublish
SO  - J Neurochem. 1999 Oct;73(4):1765-8. doi: 10.1046/j.1471-4159.1999.731765.x.