PMID- 10500279 OWN - NLM STAT- MEDLINE DCOM- 19991123 LR - 20220420 IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 64 IP - 1 DP - 1999 Oct TI - Molecular analysis of barley yellow mosaic virus isolates from China. PG - 13-21 AB - The complete sequences of both RNAs of an isolate of barley yellow mosaic virus from Yancheng, Jiangsu province, China, were determined. The sequences resembled those of an isolate from Japan (96.8% identical nucleotides for RNA1; 95.7% for RNA2) more closely than one from Germany (93.9 and 91.0%, respectively). The greatest differences between the Chinese and Japanese isolates were in the 5'-UTRs of RNAs 1 and 2 (88.9 and 91.6% identical nucleotides, respectively) and there were also some other regions of difference in P1 (RNA2) and P3, CI, NIa and the 5' end of the coat protein (CP) (RNA1). Molecular differences between isolates from ten sites widely distributed in Eastern China were studied by sequencing RNA regions coding for the CP (RNA1) and the N-terminus of the P2 protein (RNA2). The P2 fragment was more variable than the CP, and phylogenetic analysis of both regions showed that Asian and European isolates formed distinct clusters. Differences between isolates were also revealed by single-strand conformation polymorphism of reverse transcription-polymerase chain reaction products, spanning the full lengths of both RNA1 and RNA2. However, molecular variations between isolates could not be linked to earlier results showing differences in cultivar response. FAU - Chen, J AU - Chen J AD - Virology Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou, People's Republic of China. FAU - Shi, N AU - Shi N FAU - Cheng, Y AU - Cheng Y FAU - Diao, A AU - Diao A FAU - Wilson, T M AU - Wilson TM FAU - Antoniw, J F AU - Antoniw JF FAU - Adams, M J AU - Adams MJ LA - eng SI - GENBANK/AJ132268 SI - GENBANK/AJ132269 SI - GENBANK/AJ224619 SI - GENBANK/AJ224620 SI - GENBANK/AJ224621 SI - GENBANK/AJ224622 SI - GENBANK/AJ224623 SI - GENBANK/AJ224624 SI - GENBANK/AJ224625 SI - GENBANK/AJ224626 SI - GENBANK/AJ224627 SI - GENBANK/AJ224628 SI - GENBANK/AJ224629 SI - GENBANK/AJ224630 SI - GENBANK/AJ224631 SI - GENBANK/AJ224632 SI - GENBANK/AJ224633 SI - GENBANK/AJ224634 SI - GENBANK/AJ224635 SI - GENBANK/AJ224636 SI - GENBANK/AJ224637 SI - GENBANK/AJ224638 PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (RNA, Viral) SB - IM MH - Base Sequence MH - Capsid/genetics MH - China MH - Hordeum/*virology MH - Japan MH - Phylogeny MH - Potyvirus/classification/*genetics/isolation & purification MH - RNA, Viral/chemistry/genetics MH - Sequence Alignment EDAT- 1999/09/29 00:00 MHDA- 1999/09/29 00:01 CRDT- 1999/09/29 00:00 PHST- 1999/09/29 00:00 [pubmed] PHST- 1999/09/29 00:01 [medline] PHST- 1999/09/29 00:00 [entrez] AID - S0168-1702(99)00076-3 [pii] AID - 10.1016/s0168-1702(99)00076-3 [doi] PST - ppublish SO - Virus Res. 1999 Oct;64(1):13-21. doi: 10.1016/s0168-1702(99)00076-3.