PMID- 10500253
OWN - NLM
STAT- MEDLINE
DCOM- 19991105
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1447
IP  - 1
DP  - 1999 Oct 6
TI  - Molecular analysis of the GCF gene identifies revisions to the cDNA and amino
      acid sequences(1).
PG  - 125-31
AB  - GC factor (GCF) was reported as a transcriptional regulator that binds to a
      specific GC-rich sequence in the epidermal growth factor receptor (EGFR) gene
      promoter and represses its expression. In this paper, we present the data on
      three revisions of the cDNA sequence that lead to significant changes of the
      amino acid sequences of the published GCF. Firstly, 5'-rapid amplification of
      cDNA end (5'-RACE) analysis revealed that the 308 nucleotides of 5'-end of the
      previously published GCF cDNA does not exist at the 5'-end of the RACE product.
      Simultaneously, the correct 5'-end cDNA sequence of 31 nucleotides was
      identified. Secondly, the 'T' at the position 787 of the published GCF cDNA was
      not observed. Finally, a new sequence of 114 nucleotides was identified between
      the positions 851 and 852 of the published cDNA sequence. The revisions result in
      a GCF cDNA of 2661 nucleotides that encodes a protein of 781 amino acids,
      replacing the highly basic region of the amino-terminus of the published GCF with
      a new sequence of 147 amino acids. In this era of massive gene cloning and
      sequencing, this study is a warning to the biological research of recent years.
FAU - Takimoto, M
AU  - Takimoto M
AD  - Division of Gene Regulation, Cancer Institute, Hokkaido University, School of
      Medicine, N15, W7, Kita-Ku, Sapporo, Hokkaido, Japan. takimoto@med.hokudai.ac.jp
FAU - Mao, P
AU  - Mao P
FAU - Wei, G
AU  - Wei G
FAU - Yamazaki, H
AU  - Yamazaki H
FAU - Miura, T
AU  - Miura T
FAU - Johnson, A C
AU  - Johnson AC
FAU - Kuzumaki, N
AU  - Kuzumaki N
LA  - eng
SI  - GENBANK/AB026911
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA, Complementary)
RN  - 0 (GCFC2 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - DNA, Complementary/chemistry
MH  - Humans
MH  - Leukocytes/metabolism
MH  - Molecular Sequence Data
MH  - RNA, Messenger/chemistry
MH  - Repressor Proteins/chemistry/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - S016747819900127X [pii]
AID - 10.1016/s0167-4781(99)00127-x [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Oct 6;1447(1):125-31. doi:
      10.1016/s0167-4781(99)00127-x.