PMID- 10500252
OWN - NLM
STAT- MEDLINE
DCOM- 19991105
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1447
IP  - 1
DP  - 1999 Oct 6
TI  - Assignment of chromosomal locus and evidence for alternatively spliced mRNAs of a
      human sperm membrane protein (hSMP-1).
PG  - 119-24
AB  - The gene (HSD-1) coding a human sperm membrane protein (hSMP-1) was isolated from
      a human testis cDNA expression library using antibodies found in the serum of an 
      infertile woman. HSD-1 was localized to a single locus on chromosome 9 and
      assigned to band 9p12-p13 by fluorescent in situ hybridization (FISH) mapping and
      DAPI (4,6-diamidino-2-phenylindole) banding, using rat/human somatic cell hybrids
      and metaphase chromosomes of human lymphocytes. In rescreening a testis
      lambdagt10 cDNA expression library, the full-length cDNA (HSD-1) and several
      truncated cDNAs with heterologous regions were isolated from positive clones. The
      heterology consisted of deletion, insertion and alteration of the 5'-end. These
      heterologous truncated fragments may be produced by alternative splicing of
      mRNAs. Two recombinant prokaryotic expression vectors were constructed with one
      of the heterologous fragment (clone #26) with and without the alternative 5'-end.
      Escherichia coli transfected with the construct containing the alternative 5'-end
      failed to produce the recombinant product, whereas those transfected with the
      vector lacking the 5'-end produced hSMP-1. DNASIS analysis of the structure of
      #26 mRNA suggests that the 5'-end has a stable secondary configuration that may
      maintain the mRNA in an inactivated state, whereby hindering its translation and 
      preventing the expression of the gene.
FAU - Wang, H
AU  - Wang H
AD  - National Laboratory of Medical Molecular Biology, Institute of Basic Medical
      Sciences, Faculty of Basic Medicine, Chinese Academy of Medical Sciences, Peking 
      Union Medical College, Beijing, PR China.
FAU - Miao, S
AU  - Miao S
FAU - Chen, D
AU  - Chen D
FAU - Wang, L
AU  - Wang L
FAU - Koide, S S
AU  - Koide SS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Antigens, Surface)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SPAG8 protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Surface
MH  - Base Sequence
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Consensus Sequence
MH  - DNA, Complementary/isolation & purification
MH  - Escherichia coli/genetics
MH  - Gene Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/metabolism/ultrastructure
MH  - Male
MH  - Membrane Proteins/*genetics
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - RNA, Messenger/chemistry/genetics
MH  - Testis/metabolism
MH  - Transfection
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - S0167-4781(99)00136-0 [pii]
AID - 10.1016/s0167-4781(99)00136-0 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Oct 6;1447(1):119-24. doi:
      10.1016/s0167-4781(99)00136-0.