PMID- 10500246
OWN - NLM
STAT- MEDLINE
DCOM- 19991105
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1447
IP  - 1
DP  - 1999 Oct 6
TI  - Molecular cloning of the cDNA and promoter sequences for the mouse
      sodium-hydrogen exchanger regulatory factor.
PG  - 71-6
AB  - The Na/H exchanger regulatory factor (NHE-RF) was first identified as a co-factor
      for cAMP dependent protein kinase regulation of the rabbit epithelial Na/H
      exchanger. Subsequently, this protein which contains two PDZ motifs, was shown to
      interact with multiple cellular targets. To understand more fully the function of
      NHE-RF and its regulation, we have cloned the full-length cDNA for mouse NHE-RF
      and a portion of the mouse gene containing the promoter elements. NHE-RF cDNA,
      isolated from a mouse kidney cDNA library, predicted a polypeptide of 356 amino
      acids that shares striking sequence conservation within the two PDZ domains and
      in-vitro phosphorylation sites with the human and rat homologs. The nucleotide
      sequence 5' of the transcription start site, identified by primer extension
      analysis, was highly 'GC' rich and lacked canonical TATA or CAAT sequences. Using
      a luciferase reporter construct, deletion analyses localized the critical segment
      for gene expression in mouse medullary thick ascending limb cells to 114 bp 5' of
      the transcription start site. Although NHE-RF has been recently identified as an 
      estrogen-inducible gene, the lack of an estrogen-response element in the mouse
      NHE-RF 5'-non-coding-sequence and the inability to demonstrate estrogen
      stimulation of reporter gene expression in MCF-7 cells suggests a
      non-conventional or indirect mechanism for NHE-RF regulation by estrogen.
FAU - Weinman, E J
AU  - Weinman EJ
AD  - Department of Medicine, West Virginia University School of Medicine, 1 Medical
      Center Drive, Morgantown, WV, USA. eweinman@wvudeptmed1.hsc.wvu.edu
FAU - Steplock, D
AU  - Steplock D
FAU - Zhang, X
AU  - Zhang X
FAU - Akhter, S
AU  - Akhter S
FAU - Shenolikar, S
AU  - Shenolikar S
LA  - eng
SI  - GENBANK/U74079
GR  - DK37319/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA, Complementary)
RN  - 0 (Estrogens)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sodium-Hydrogen Exchangers)
RN  - 0 (sodium-hydrogen exchanger regulatory factor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry
MH  - Estrogens/pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Kidney/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phosphoproteins/chemistry/*genetics
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - Restriction Mapping
MH  - Sequence Alignment
MH  - Sodium-Hydrogen Exchangers/antagonists & inhibitors
MH  - Transcription, Genetic/drug effects
EDAT- 1999/09/29 00:00
MHDA- 1999/09/29 00:01
CRDT- 1999/09/29 00:00
PHST- 1999/09/29 00:00 [pubmed]
PHST- 1999/09/29 00:01 [medline]
PHST- 1999/09/29 00:00 [entrez]
AID - S0167-4781(99)00100-1 [pii]
AID - 10.1016/s0167-4781(99)00100-1 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Oct 6;1447(1):71-6. doi:
      10.1016/s0167-4781(99)00100-1.